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Pollen Quantity, Viability and in vitro Pollen Germination of Longan (Dimocarpus longan Lour.)‏.

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Title Pollen Quantity, Viability and in vitro Pollen Germination of Longan (Dimocarpus longan Lour.)‏.
Not Available
 
Creator Gupta A K
Singh M
Marboh E S
Nath V
Pongener A
Anal A K D
 
Subject Longan
Pollen
Viability
Germination
 
Description Not Available
Dimocarpus longan Lour. is an evergreen fruit tree species of Sapindaceae family grown
in subtropical region. Short flowering period coupled with narrow genetic base are the
major constraints in longan genetic improvement. Pollens are known to directly influence
reproductive success and genetic structure of the plant population. In this study, we
compared the pollen quantity, viability and in vitro pollen germination of M1 and M2 type
flowers of nine longan genotypes. Acetocarmine, 2, 3, 5-triphenyl tetrazolium chloride, 2,
5-diphenyl monotetrazolium bromide and aniline blue-lectophenol staining methods were
used for pollen viability assessment while different concentrations of sucrose, boric acid
(H3BO3) and agar were used for in vitro pollen germination test through hanging drop
technique. Pollen tube growth was evaluated by incubating the media containing pollen at
different temperature and duration. Highest pollen quantity per anther (5278) was observed
in M2 flower of Lgc-06. Acetocarmine (2.0 %) showed best results for pollen viability
(94.37 %) in M2 pollen of Lgc-06. Germination media containing 15 % sucrose + 100
ppm Boric acid + 0.5 % agar showed promising results for pollen germination (80.33 %).
Highest germination rate (83.66 %) and maximum pollen tube growth was observed at
30oC for 24 hours. Overall, pollen from M2 flower had significantly higher pollen
quantity, viability and in vitro pollen germination percentage compared to pollen from M1
flower.
Not Available
 
Date 2018-11-09T06:39:21Z
2018-11-09T06:39:21Z
2017-07-10
 
Type Research Paper
 
Identifier Gupta, A.K., Singh, M., Marboh, E.S., Nath, V., Pongener, A. and Anal,‏ A.K.D. 2017. Pollen Quantity, Viability and in vitro Pollen Germination of Longan (Dimocarpus longan Lour.)‏. Int. J. Curr. Microbiol. App. Sci. 6(7): 270-278.
2319-7706
http://krishi.icar.gov.in/jspui/handle/123456789/9909
 
Language English
 
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