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Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis

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Title Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis
 
Creator Prathibha VH, Hegde Vinayaka, Sharadraj K.M, Rajesh M.K, Rachana K.,E and Chowdappa P.
 
Subject Coconut . Arecanut palm . Phytophthora . HRM analysis . Rapid detection, PCR
 
Description Not Available
Diseases caused by closely related species of Phytophthora such as P. palmivora, P. nicotianae, P. capsici, P. meadii, P. citrophthora and P. colocasiae are major production constraints of plantation crops in India. A reliable method for the rapid identification of these Phytophthora species is required for production of disease-free planting material and promote exports. A specific PCR assay with primer pairs designed from the ITS region effectively differentiated Phytophthora species belonging to different clades viz., P. palmivora, P. nicotianae and P. capsici. In order to differentiate closely related species (P. meadii, P.
citrophthora and P. colocasiae), a high-resolution melting (HRM) curve analysis, using primers designed based on single nucleotide differences in A/Tand C/A alleles was developed and validated. HRMcurve analysis proved to be a fast and accurate technique for differentiation of closely related species of Phytophthora.
 
Date 2018-11-24T08:00:39Z
2018-11-24T08:00:39Z
2018-05-01
 
Type Research Paper
 
Identifier Prathibha V.H. et.al 2018. Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis Journal of Plant Pathology 100(2): 233-240
http://krishi.icar.gov.in/jspui/handle/123456789/13080
 
Language English
 
Publisher Journal of Plant Pathology, vol.100 (2): 233-240