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Detection of Root (Wilt) Disease of Coconut (Cocos nucifera L.) based on polyacrylamide gel electrophoresis

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Title Detection of Root (Wilt) Disease of Coconut (Cocos nucifera L.) based on polyacrylamide gel electrophoresis
Not Available
 
Creator Chaturvedi, V. K.
Rajeev, G.
Nampoothiri, C. K.
 
Subject root (wilt) disease
coconut
detection
SDS PAGE
 
Description Not Available
Root (wilt) disease (RWD), caused by phytoplasma, is a major problem causing decreased coconut productivity in southern districts of Kerala and its bordering districts of Tamil Nadu in India. The disease is non-curable but its incidence can be reduced by propagating seedlings from nuts of disease free palms. The disease free palms are selected by ELISA test which uses antiserum obtained from rabbits against purified phytoplasma extract containing 29, 28 and 18.5 K Da proteins. With an objective of developing a simpler and easier biochemical test than ELISA for RWD detection in coconut , direct SDS PAGE profiles of soluble proteins from crude leaf extracts of healthy and diseased palms of West Coast Tall (susceptible) , Chowghat Green Dwarf and Malayan Green Dwarf (high degree resistant) cultivars were evaluated for differences in intensities of protein bands with molecular masses corresponding closest to the purified phytoplasma extract proteins. It was found that the 31.2, 37.3, 16.9 and 13.8 KDa bands in WCT cultivar, 31 and 40.6 KDa in CGD cultivar and 29.9 and 37.1 KDa bands in the MGD cultivar showed consistent differences in intensities and/or presence or absence of certain bands between healthy and diseased palms. Correlations and path analysis relationship between intensity of different protein bands and ELISA value also showed significant association of one or two of these marker bands with ELISA values in each cultivar. The SDS PAGE profiles of crude leaf extracts could be used to effectively distinguish healthy and diseased RWD palms in these three cultivars.
Not Available
 
Date 2018-11-27T07:09:12Z
2018-11-27T07:09:12Z
2016-01-01
 
Type Article
 
Identifier IRA-International Journal of Applied Sciences,Vol.03, Issue 03 (2016)
2455-4499
http://krishi.icar.gov.in/jspui/handle/123456789/13581
 
Language English
 
Relation Not Available;
 
Publisher Not Available