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Genetic diversity of Xanthomonas campestris pv. campestris isolated from Brassica crops using RAPD and Rep-PCR

Indian Agricultural Research Journals

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Title Genetic diversity of Xanthomonas campestris pv. campestris isolated from Brassica crops using RAPD and Rep-PCR
 
Creator SINGH, DINESH
RATHAUR, PRIYANKA SINGH
SINGH, ASHWARIYA
RAGHUWANSHI, RICHA
 
Subject Brassica, Crucifers, Genetic diversity, Rep-PCR, RAPD, X. campestris pv. campestris
 
Description Xanthomonas campestris pv. campestris (Xcc) is causal agent of black rot disease in crucifers. Genetic diversity, 28 isolates of Xcc collected from cole crops (cauliflower, cabbage, broccoli and knol khol), turnip, brown and yellow mustard crops from northern region of India was performed by using rep-PCR fingerprinting (BOX, REP and ERIC) and random amplified polymorphic DNA (RAPD). Five DNA type groups were formed by using rep-PCR fingerprinting. In RAPD study, six primers showed monomorphic bands and remaining six primers (OPB-10, OPB- 15, UBC-245, D-11, UBC-220 and OPA-11) exhibited polymorphic amplified product in all isolates of Xcc. Xcc- C281 and Xcc-C261 and Xcc-C268 were formed separate group as DNA types, 1, 2, 3, 4, 5 and 6 respectively. Genetic variability was found among the isolates of Xcc based on their hosts. They were categorized into eight groups at DNA typing at ~50% similarity coefficient and formed 8 DNA type groups at ~ 50% similarity coefficient. Eighteen isolates of Xcc were grouped under DNA type 7, whereas in DNA type 8 (4 isolates).
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2015-06-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/49230
 
Source The Indian Journal of Agricultural Sciences; Vol 85, No 6 (2015)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/49230/21101
 
Rights Copyright (c) 2015 The Indian Journal of Agricultural Sciences