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Genetic diversity of biovar 3 and 4 of Ralstonia solanacearum causing bacterial wilt of tomato using BOX- PCR, RAPD and hrp gene sequences

Indian Agricultural Research Journals

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Title Genetic diversity of biovar 3 and 4 of Ralstonia solanacearum causing bacterial wilt of tomato using BOX- PCR, RAPD and hrp gene sequences
 
Creator SINGH, DINESH
SINHA, SHWETA
CHAUDHARY, GARIMA
YADAV, D K
MONDAL, K K
 
Subject Biovar, Genetic diversity, Ralstonia solanacearum, Tomato
 
Description Genetic diversity of Ralstonia solanacearum, causal agent of bacterial wilt of tomato was assessed by using three different molecular methods such as random amplified polymorphism DNA (RAPD), BOX-PCR and hrp (hypersensitive response and pathogenicity) gene sequence analysis technique. Twelve isolates of Ralstonia solanacearum belonging to biovar 3 (9 isolates) and 4 (3 isolates) were collected from Northern parts of India including Himachal Pradesh and Uttarakhand states from infected tomato plants. Out of 16 primers used in RAPD fingerprinting, four primers (OPA-2, OPA-11, OPC-5, OPE-7) showed monomorphic bands and remaining 12 primers exhibited polymorphic amplified products of both the biovars of R. solanacearum. The primer OPE-10 showed the highest level of genetic diversity among the isolates. Ten isolates of R. solanacearum were classified into two clusters at 20 per cent similarity coefficient and cluster 1 represented all isolates of biovar 3 (UTT-23, UTT-10, UTT-26, HPT11, UTT-9, UTT-32, HPC-3) and cluster 2 comprised the biovar 4 (UTT-22, HPT-3, UTT-24). BOX-PCR fingerprint of R. solanacearum clearly distinguished biovar 3 and 4 grouped into two distinct clusters at 40% similarity coefficient. Cluster 1 represented all isolates of R. solanacearum biovar 3 and cluster 2 comprised the biovar 4 isolates. The isolates of R. solanacearum have genetic diversity in hrpB gene, but it could not differentiate the isolates of biovar 3 and 4. However, the biovars 3 and 4 of R. solanacearum can be genetically distinguished by using BOX- PCR and specific primer of RAPD.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2014-03-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/38590
 
Source The Indian Journal of Agricultural Sciences; Vol 84, No 3 (2014)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/38590/17354
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences