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Application of Immuno-diagnosis for plant viruses occurring in India

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Title Application of Immuno-diagnosis for plant viruses occurring in India
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Creator Bhat, A I, Yogitha, M.
 
Subject Antigen
Antiserum
Detection
Diagnosis
Dotimmunobinding assay
ELISA
Immunodiagnosis
PCR
Real-time PCR
Serology
virus
Lateral flow immuniassay
 
Description Not Available
Diseases caused by viruses cause heavy crop losses worldwide including India.
Correct diagnosis of viruses is the primary requirement for disease management.
Of the different methods available for diagnosis, serological methods are useful
in the detection of viruses in seeds, plants and insect vectors. The production of
virus specific antiserum is the basis for a successful serological method. The
earliest serological methods such as precipitin tests are low in sensitivity. With
the advent of enzyme linked immunosorbent assay (ELISA) and its subsequent
application to plant virus detection during 1980s, some laboratories in India also
started working on the same. During 1990s, ELISA and its variants became more
popular in many laboratories of India working on plant viruses. During the same
period Indian researchers also used other serological methods such as dot immunobinding
assay (DIBA), immunosorbent electron microscopy (ISEM), electro-blot
immunoassay (EBIA) and florescent antibody technique in the detection of
plant viruses. Immunocapture PCR (IC-PCR) which combines both serology and
PCR has also been used by many Indian researchers in the identification and
characterization of viruses infecting different crops. Lateral flow immunoassay
technology is the recent technique that offers several advantages over traditional
immunoassays, such as procedural simplicity, limited requirement for special
skills or expensive equipment, and rapid results is also gaining importance in the
country. During the last decade, recombinant antibody (rAb) engineering has
emerged as one of the promising approaches in plant virus diagnosis. rAb fragments
in all various formats (Fab, Fv and scFv) can be expressed in different systems including bacteria, insect, yeast, plant and mammalian cells, which can
be used efficiently in various format of ELISA for diagnosis of plant viruses.
India also started a modest beginning in this area now.
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Date 2019-11-30T11:23:27Z
2019-11-30T11:23:27Z
2017
 
Type Book chapter
 
Identifier Bhat, A I, Yogitha, M. 2017. Application of Immuno-diagnosis for plant viruses occurring in India. In A Century of Plant Virology in India, (B. Mandal, G.P.Rao, V K Baranwal and R.K.Jain eds.), Springer Nature Singapore Pte Ltd. DOI 10.1007/978-981-10-5672-7_26.
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http://krishi.icar.gov.in/jspui/handle/123456789/26062
 
Language English
 
Relation Not Available;
 
Publisher Bhat, A I, Yogitha, M.