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Gamma radiation induced phenotypic diversity in Dendrobium ‘Emma White’

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Title Gamma radiation induced phenotypic diversity in Dendrobium ‘Emma White’
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Creator Rubina Sherpa
Ramgopal Devadas
Suprasanna Penna
Ravi Kishore Pamarthi
T.D. Nikam
S.N. Bolbhat
 
Subject Dendrobium
Gamma radiation
Mutagenesis
Phenotypic variation
 
Description Not Available
Dendrobium, one of the largest genera of family Orchidaceae, is economically important both as an ornamental and medicinal crop. Varietal improvements of Dendrobium through the conventional breeding methods are difficult and challenging due to existence of incompatibility among the respective primary gene pool. Gamma irradiation technique is used to induce variability and generate mutants with improved traits in a short period. In our study, protocorm-like-bodies (PLBs) of Dendrobium hybrid ‘Emma White’ were exposed to gamma rays (of 10 - 80 Gy) and in vitro propagated up to M1V5 generation. Plantlets were regenerated from PLBs treated at 10, 20 and 40 Gy; while 60 and 80 Gy treated PLBs showed only proliferation even after 24 months of post-irradiation treatment. Phenotypic variations were recorded among the plantlets during the ex-vitro hardening stage. Leaf variations in terms of shape, size, structure, surface, tip and colour were observed in plantlets generated at 10 Gy. Significant variations and deformity were observed in leaf structure and arrangement at 40 Gy radiation level. Among 120 putative mutants, 12 lines viz. 10/4, 10/5, 10/12, 10/17, 10/35, 10/36, 10/60, 10/66, 10/75, 10/76, 10/82, 10/85 were selected for further analyses including molecular characterization. Mutant line 10/46 was identified with early flowering, within 09 months 24 days from the date of hardening. Findings indicate impact of gamma radiation effects on enhancing genetic variability of both vegetative and flowering traits of Dendrobium hybrid.
Board of Research in Nuclear Sciences, BARC, Mumbai
No: 35/14/22/2016-BRNS/35061 Date: 06.06.2016
 
Date 2019-11-29T08:32:01Z
2019-11-29T08:32:01Z
2019-08-26
 
Type Presentation
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/25827
 
Language English
 
Relation Souvenir cum Abstracts e-Book;176
 
Publisher Society for Biotic and Environmental Research, Tripura