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Comparative analysis of two catalytically distinct endoglucanses from Aspergillus nidulans

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Title Comparative analysis of two catalytically distinct endoglucanses from Aspergillus nidulans
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Creator Baljit Kaur, HS Oberoi, BS Chadha
 
Subject Thermoacidophilic endoglucanases, GH7, GH12, barley β-glucan
 
Description Not Available
This study reports purification and characterization of two catalytically distinct endoglucanases (EGI and EGII)
from a thermotolerant fungus Aspergillus nidulans. The endoglucanases (EGI and EGII) exhibited molecular
masses of 56 and 31 kDa and pIs of 3.6 and 3.8, respectively. EGI was putatively classified as GH7 family
member catalyzed carboxymethyl cellulose, xyloglucan, barley β-glucan as well as pNP-β-D-lactopyranoside and
pNP-cellobioside, and was optimally active at 50°C and pH 4.0. Whereas, EGII lacking CBD preferentially
recognized barley β-glucan when compared substrate CMC, xyloglucan and lichenan and was putatively
classified as GH12 member. Interestingly, EGII was characterized to be thermoacidophilic exhibiting 96% its
activity at pH 2.0 and at 60 °C. Hydrolysis of barley β-glucan and CMC by EGI and EGII liberated cellobiose as
a major product. HPLC analysis showed that barley β-glucan hydrolysate obtained by action of EGI showed high levels of glucose in addition to cellobiose indicating towards an exo type action of this enzyme.
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Date 2019-10-19T10:08:00Z
2019-10-19T10:08:00Z
2015-04-27
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/23679
 
Language English
 
Relation Not Available;
 
Publisher Not Available