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Microsatellite markers analysis for evaluation of genetic variation in mango genotypes

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Title Microsatellite markers analysis for evaluation of genetic variation in mango genotypes
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Creator Kamlesh Kumar, Manish Srivastav, Sanjay Kumar Singh, Vinod and Chet Ram
 
Subject Microsatellite markers, genetic diversity, mango, PCR, PIC
 
Description Not Available
The study was under taken to evaluate the genetic variation in seven mango genotypes, namely,
Amrapali, Dushehari, Janardan Pasand, Neelam, Pusa Arunima, Sensation and Tommy Atkins using SSR
markers. At first, forty-two microsatellite markers were screened in mango genotypes for getting
polymorphic SSR markers then polymorphic SSR markers were utilized for further analysis. Out of 42
primer pairs screened, 13 primer pairs namely (LMMA2, LMMA3, LMMA4, LMMA7, LMMA8,
LMMA9, LMMA11, LMMA12, LMMA16 and ESTD1, ESTD2, ESTD9 and ESTD10) were found
polymorphic and rest of primers got monomorphic status. These 13 polymorphic markers were generated
43 alleles varied from 2 to 5 with an average of 3.30 alleles per locus among the mango genotype tested.
The major allele frequency was varied from 0.36 to 0.86 for the locus ESTD9 and LMMA4, respectively.
Mean major allele frequency was 0.60 for all the polymorphic loci. The observed heterozygosity (Ho) for
individual loci ranged from 0.14 (ESTD2) to 0.86 (LMMA16), with an average of 0.44 over all loci.
Whereas, gene diversity varied from 0.24 (LMMA4) to 0.74 (ESTD9), with an average of 0.53.The PIC
value ranged from 0.21 (LMMA4) to 0.70 (ESTD9) with an average of 0.47. The microsatellite markers
study helped in identification of genetic distance among mango genotypes. Dissimilarity matrix
suggested a clear separation between Indigenous and exotic genotypes.
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Date 2020-05-21T05:33:24Z
2020-05-21T05:33:24Z
2019-04-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/35963
 
Language English
 
Relation Not Available;
 
Publisher International Journal of Chemical Studies