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Phytobeneficial root endophytes of root (wilt) disease resistant coconut palm

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Title Phytobeneficial root endophytes of root (wilt) disease resistant coconut palm
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Creator Indhuja S, Anju R, Merin Babu, A.A.Haris, Krishnakumar, V.
 
Subject endophytes, Bacillus
 
Description Not Available
Phytobeneficial root endophytes of root (wilt) disease resistant coconut palm
Indhuja S1*, Anju R2, Merin Babu1, A.A.Haris1, Krishnakumar, V1
1ICAR-Central Plantation Crops Research Institute, Regional Station, Kayamkulam, Krishnapuram P.O., Alappuzha-690 533, Kerala, India
2Department of Microbiology, St. Mary’s College for women, Paliakkara, Thiruvalla
*Corresponding author

Plant-endophytes interaction and its beneficial role in host fitness and stress mitigation is well established. Endophytes represent a selected fraction of microbial communities of diverse heterotrophs inhabiting the rhizosphere. Endophytic bacteria colonize the roots in high numbers and often exhibit systemic occurrence in the plants without causing negative impact. Plant growth promotion, defense response elicitation and stress modulation are a few functional responsibilities evinced in plant-endophytes interaction. This study is attempted to understand the plant growth promoting functions of culturable bacterial root endophytes of root (wilt) disease (RWD) resistant coconut palm. Fresh feeding roots of apparently healthy (field resistant) coconut selected based on RWD indexing method were sampled for bacterial root endophytes isolation. Plate count showed an average of 2 x 103 cfu/g of fresh root sample on trypticase soya agar. Seventeen bacteria with distinct colony morphotypes were screened for phytobeneficial traits. Of the seventeen, 9 bacteria produced indoleacetic acid ranging from 0.2 to 4.7 µg/mL. Qualitative assessment of nitrogen fixing ability and aminocyclopropane carboxylate (ACC) deaminase activity were made based on their growth on respective media. Six of 17 bacteria grew on nitrogen (N) free media indicating their N fixing potential among which 4 were IAA producers. Seven isolates utilized ACC as their sole N source indicative of their ACC deaminase activity, of which 4 had nitrogen fixation and 3 had IAA production potential. All the 17 bacterial isolates showed growth compatibility with the biocontrol agent Trichoderma harzianum CPTD-28. Three isolates CRE2, CRE9 and CRE15 with all the above plant growth promoting traits were selected for further characterization. All the three bacteria were Gram positive rods with indole negative and catalase positive reactions. Based on 16S rRNA sequence analysis, CRE 2 and CRE 9 were identified belonging to Bacillus subtilis. CRE 15 showed a close similarity with Bacillus Shackletonii. Studies on endophytes of RWD resistant coconut palm in RWD tract are limited and remain a prospective strategy for root (wilt) disease management of coconut.
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Date 2019-11-14T14:54:16Z
2019-11-14T14:54:16Z
2019-03-01
 
Type Proceedings
 
Identifier Indhuja S, Anju R, Merin Babu, A.A.Haris, Krishnakumar, V. 2019. Phytobeneficial root endophytes of root (wilt) disease resistant coconut palm In: Book of Abstracts: Placrosym 23- Climate Resilient Technologies for Plantation Crops, Central Coffee Research Institute, Chikkamagaluru. P.no. 123
978-81-932263-8-4
http://krishi.icar.gov.in/jspui/handle/123456789/24723
 
Language English
 
Relation Not Available;
 
Publisher CCRI, Chikkamagaluru, Karnataka