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Callus induction, somatic embryogenesis, in vitro plantlet development and ex vitro transplantation of two date palm (Phoenix dactylifera L.) cultivars

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Title Callus induction, somatic embryogenesis, in vitro plantlet development and ex vitro transplantation of two date palm (Phoenix dactylifera L.) cultivars
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Creator Kamlesh Kumar, Dhurendra Singh and PL Saroj
 
Subject Somatic embryogenesis, in vitro plantlet development, ex vitro transplantation, date palm cultivars
 
Description Not Available
The protocol has been developed and standardized in two date palm (Phoenix dactylifera L.) cultivars.
Protocol was cultivar and plant growth regulators dependant. Embryogenic callus from excised shoot tips
was induced on MS medium supplemented with 2,4-D 100 mg/l. A combination of ά-naphthalene acetic
acid and 6-benzyladenine (NAA 0.1 + BA 0.05) in MS medium multiplied callus 3-4 times followed by
abscisic acid (ABA, 0.5 mg/l) treatment matures callus and accelerated the initiation of somatic embryos
in both the cultivars. Treatment combination of abscisic acid (0.5 mg/l) + indole-3-butyric acid (IBA,
0.05 mg/l) in medium found effective in maturation of somatic embryos. Somatic embryos germinated
and converted into plantlets in half strength modified MS medium containing different concentrations of
IBA @ 0.1, 0.2, 0.3 mg/l and NAA @ 0.1, 0.2, 0.3 mg/l. At a higher concentration (0.3 mg/l), in vitro
roots and leaves (plantlets) were developed while at lower concentrations, response was comparatively
poor. Secondary hardened plants after primary hardening were transplanted in the field for assessing their
field performance. The findings of this paper indicate that enhanced production of somatic embryos and
their conversion into plantlets can be a potential source of alternative mass propagation of date palm.
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Date 2021-01-21T04:12:51Z
2021-01-21T04:12:51Z
2020-01-11
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/44912
 
Language English
 
Relation Not Available;
 
Publisher Not Available