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De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry

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Title De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry
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Creator Johnson K. George
Sreekumar Shelvy
Abdulkabeer Muhammed Fayad
Palaniyandi Umadevi
U. B. Angadi
Mir Asif Iquebal
Sarika Jaiswal
Anil Rai
Dinesh Kumar
 
Subject PnTPS
Denovo transcriptome assembly
Berry transcriptome
Terpenoid pathway
Terpenes
Piper nigrum
 
Description Not Available
Though the volatile profiles of black pepper have been reported already, the information on terpene synthase family genes is not known. In this study, using a combinatorial approach, the berry hybrid transcriptome assembly of llumina and nanopore sequencing, the entire terpene synthase family responsible for the biosynthesis of the flavor-imparting volatiles in black pepper berries was profiled. The profile shows 98 terpene synthases from various terpene synthesis pathways. Three important monoterpene synthases were also validated by targeted amplification, sequencing and homology modeling. This study provides the first of its kind information on the terpene synthase family profile in Piper nigrum, which is potentially a major step for further characterization of the functional terpene synthase genes in black pepper.
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Date 2022-03-15T06:36:16Z
2022-03-15T06:36:16Z
2021-05-04
 
Type Research Paper
 
Identifier George, J.K., Shelvy, S., Fayad, A.M. et al. De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry. Physiol Mol Biol Plants 27, 1153–1161 (2021). https://doi.org/10.1007/s12298-021-00986-4
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http://krishi.icar.gov.in/jspui/handle/123456789/70230
 
Language English
 
Relation Not Available;
 
Publisher Not Available