De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry
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Title |
De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry
Not Available |
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Creator |
Johnson K. George
Sreekumar Shelvy Abdulkabeer Muhammed Fayad Palaniyandi Umadevi U. B. Angadi Mir Asif Iquebal Sarika Jaiswal Anil Rai Dinesh Kumar |
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Subject |
PnTPS
Denovo transcriptome assembly Berry transcriptome Terpenoid pathway Terpenes Piper nigrum |
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Description |
Not Available
Though the volatile profiles of black pepper have been reported already, the information on terpene synthase family genes is not known. In this study, using a combinatorial approach, the berry hybrid transcriptome assembly of llumina and nanopore sequencing, the entire terpene synthase family responsible for the biosynthesis of the flavor-imparting volatiles in black pepper berries was profiled. The profile shows 98 terpene synthases from various terpene synthesis pathways. Three important monoterpene synthases were also validated by targeted amplification, sequencing and homology modeling. This study provides the first of its kind information on the terpene synthase family profile in Piper nigrum, which is potentially a major step for further characterization of the functional terpene synthase genes in black pepper. Not Available |
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Date |
2022-03-15T06:36:16Z
2022-03-15T06:36:16Z 2021-05-04 |
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Type |
Research Paper
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Identifier |
George, J.K., Shelvy, S., Fayad, A.M. et al. De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry. Physiol Mol Biol Plants 27, 1153–1161 (2021). https://doi.org/10.1007/s12298-021-00986-4
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/70230 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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