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Delineation of molecular interactions of plant growth promoting bacteria induced β-1,3-glucanases and guanosine triphosphate ligand for antifungal response in rice: a molecular dynamics approach

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Title Delineation of molecular interactions of plant growth promoting bacteria induced β-1,3-glucanases and guanosine triphosphate ligand for antifungal response in rice: a molecular dynamics approach
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Creator Yachana Jha
Budheswar Dehury
S. P. Jeevan Kumar
Anurag Chaurasia
Udai B. Singh
Manoj Kumar Yadav
U. B. Angadi
Rajiv Ranjan·
Minaketan Tripathy
R. B. Subramanian
Sunil Kumar
Jesus Simal‑Gandara
 
Subject Biotic stress
Dot blot analysis
β-1,3-glucanases
GTP ligand
Molecular dynamics simulation
 
Description Not Available
Background The plant growth is infuenced by multiple interactions with biotic (microbial) and abiotic components in their
surroundings. These microbial interactions have both positive and negative efects on plant. Plant growth promoting bacterial
(PGPR) interaction could result in positive growth under normal as well as in stress conditions.
Methods Here, we have screened two PGPR’s and determined their potential in induction of specifc gene in host plant to
overcome the adverse efect of biotic stress caused by Magnaporthe grisea, a fungal pathogen that cause blast in rice. We
demonstrated the glucanase protein mode of action by performing comparative modeling and molecular docking of guanosine
triphosphate (GTP) ligand with the protein. Besides, molecular dynamic simulations have been performed to understand the
behavior of the glucanase-GTP complex.
Results The results clearly showed that selected PGPR was better able to induce modifcation in host plant at morphological,
biochemical, physiological and molecular level by activating the expression of β-1,3-glucanases gene in infected host plant.
The docking results indicated that Tyr75, Arg256, Gly258, and Ser223 of glucanase formed four crucial hydrogen bonds
with the GTP, while, only Val220 found to form hydrophobic contact with ligand.
Conclusions The PGPR able to induce β-1,3-glucanases gene in host plant upon pathogenic interaction and β-1,3-glucanases
form complex with GTP by hydrophilic interaction for induction of defense cascade for acquiring resistance against Mag naporthe grisea.
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Date 2022-05-30T06:00:57Z
2022-05-30T06:00:57Z
2021-12-07
 
Type Research Paper
 
Identifier ha, Y., Dehury, B., Kumar, S.P.J. et al. (2022). Delineation of molecular interactions of plant growth promoting bacteria induced β-1,3-glucanases and guanosine triphosphate ligand for antifungal response in rice: a molecular dynamics approach. Mol Biol Rep 49, 2579–2589. https://doi.org/10.1007/s11033-021-07059-5
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http://krishi.icar.gov.in/jspui/handle/123456789/72408
 
Language English
 
Relation Not Available;
 
Publisher Springer