Identification and validation of candidate genes for high calcium content in finger millet [Eleusine coracana (L.) Gaertn.] through genome-wide association study
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Title |
Identification and validation of candidate genes for high calcium content in finger millet [Eleusine coracana (L.) Gaertn.] through genome-wide association study
Not Available |
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Creator |
Divya Sharma
Apoorv Tiwari Salej Sood P.K. Meher Anil Kumar |
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Subject |
Single nucleotide polymorphism
Genome-wide association mapping Finger millet Grain calcium |
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Description |
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Calcium is one of the most important mineral elements required in adequate amounts in the diet for sound health. Improved intake of calcium in the diet is the most cost-effective way to overcome its deficiency. Finger millet [Eleusine coracana (L.) Gaertn.] is an excellent model system for investigating the genetic control of calcium accumulation in crops because of the very high calcium content in its grains. Therefore, a set of 202 diverse global finger millet accessions were evaluated for grain calcium content in two diverse environments in India. Phenotypic measurements for grain calcium content showed wide variation among the accessions, which ranged from 53 to 454 mg per 100g grains. High throughput genotyping by sequencing (GBS) identified 2977 genome-wide high-quality SNPs for marker-trait association (MTA) analysis with respect to grain calcium content, days to maturity, and grain yield. We found 44 common SNP-trait associations using general and mixed linear models contributing 4.80–17.79% phenotypic variance for calcium trait, 5 and 1 common SNPs associated with grain yield and days to maturity expressing 5.52–13.12%, and 5.83–6.75% phenotypic variance, respectively. Among 44 MTA's for calcium content, two putative SNP marker sequences were found homologous to candidate genes viz., calmodulin-binding protein (CBP) and CBL-Interacting Protein Kinase7 (CIPK7) of Setaria italic (Foxtail millet), which might be playing a pivotal role in grain calcium accumulation. Differential expression analysis by qPCR showed that the two genes (EcCBP and EcCIPK7) were highly expressed in high calcium genotypes (HCG) as compared to medium and low calcium genotypes (LCG). These two genes might be involved in regulating the activities of CaX exchanger and calmodulin (CAM) proteins resulting in high grain calcium accumulation. Not Available |
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Date |
2022-08-07T06:41:45Z
2022-08-07T06:41:45Z 2022-09-01 |
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Type |
Research Paper
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Identifier |
Divya Sharma, Apoorv Tiwari, Salej Sood, P.K. Meher, Anil Kumar(2022). Identification and validation of candidate genes for high calcium content in finger millet [Eleusine coracana (L.) Gaertn.] through genome-wide association study, Journal of Cereal Science, 107, 103517, ISSN 0733-5210, https://doi.org/10.1016/j.jcs.2022.103517.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/73734 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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