Cryopreservation of apple (Malus domestica 'Benoni') dormant buds using two-step freezing method
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Title |
Cryopreservation of apple (Malus domestica 'Benoni') dormant buds using two-step freezing method
Not Available |
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Creator |
S Gupta
J I Mir |
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Subject |
Ex situ
Conservation Malus spp Cryopreservation, Winter Germplasm |
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Description |
Not Available
Apple is the most important temperate fruit in India. The top apple producing states are Jammu and Kashmir (J&K), Himachal Pradesh (HP) and Uttarakhand (UK). The apple germplasm is mainly conserved in the field genebanks at the ICAR-National Bureau of Plant Genetic Resource s Regional Station, Shimla (HP) and Bhowali (UK); ICAR-Central Institute of Temperate Horticulture, Srinagar (J&K); ICAR-Indian Agricultural Research Institute s Regional Station, Shimla (HP); Dr. Y.S. Parmar University of Horticulture and Forestry, Solan (HP); Govind Ballabh Pant University of Agriculture and Technology, Pantnagar (UK); Sher-e-Kashmir University of Agricultural Science and Technology, Srinagar (J&K). To have a backup, some accessions are being maintained under in vitro genebank at New Delhi. For long-term conservation of apple accessions, cryopreservation is the only method. Dormant buds of apple (Malus domestica Benoni ) were cryopreserved by two-step freezing method. Apple twigs were collected from Srinagar at the sub-freezing temperature. Twigs were stored at 5-6°C until use. The buds were scooped from the twigs and desiccated on silica gel. Then the buds were subjected to gradual low temperature treatments up to -30°C before submerging into liquid nitrogen. Programmable freezer was not used for freezing treatments. Post-thaw recovery (in vitro) was about 20%. The average rate of shoot formation was about 80%. This protocol of two-step freezing method was successfully applied to other three apple cultivars with the post-thaw in vitro recovery about 11-40%. This method appears to be a promising technique for cryopreserving dormant buds from field grown apple trees. Not Available |
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Date |
2020-08-19T10:14:06Z
2020-08-19T10:14:06Z 2019-01-01 |
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Type |
Research Paper
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Identifier |
Gupta, S. and Mir, J.I. (2019). Cryopreservation of apple (Malus domestica 'Benoni') dormant buds using two-step freezing method. Acta Hortic. 1234, 323-328
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/39813 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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