Application of automated mini–Solid-Phase Extraction cleanup for the analysis of pesticides in complex spice matrixes by GC-MS/MS
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Title |
Application of automated mini–Solid-Phase Extraction cleanup for the analysis of pesticides in complex spice matrixes by GC-MS/MS
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Creator |
Goon A., Shinde R., Ghosh B, Banerjee Kaushik
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Subject |
Automated mini–solid-phase extraction cleanup
Pesticide residue analysis in spices GC-MS/MS |
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Description |
Not Available
Abstract Background: Pesticide residues are routinely tested in spices for trade compliance. This results in a huge sample load for food testing laboratories and demands automation in sample preparation. Although there exists a method for the analysis of pesticides in fruits using an automated sample cleanup by mini–solid-phase extraction (mini-SPE) technique, no study is available to date on spices. Objective: This study aims to develop an automated sample cleanup method using mini-SPE technique in a range of spices, including chili powder, turmeric, black pepper, cumin, coriander, and cardamom. Methods: This automated sample preparation workflow involved an X-Y-Z instrument autosampler, and a set of mini-SPE cartridges comprising cleanup sorbents. Spice samples were extracted by acetonitrile, and the extract was put into an autosampler vial for automated mini-SPE cleanup before analysis by GC tandem MS. For an efficient cleanup, three different sorbent compositions were compared along with various automated workflows. Results: For the relatively simple matrixes (e.g., coriander, cumin, and cardamom), the LOQ for the target pesticides was 10 ng/g with acceptable recovery, and precision. The method provided an LOQ of 10 ng/g for around 77% of the compounds in the relatively complex matrixes (e.g., turmeric, chili powder, and black pepper). The remainder of the compounds had satisfactory recoveries at 20 ng/g and higher levels. Conclusions: Given its time effectiveness and efficient analytical performance, this method can be adopted in commercial food testing laboratories for time-bound analysis of a large volume of samples. Not Available |
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Date |
2020-07-31T06:20:47Z
2020-07-31T06:20:47Z 2020-01-29 |
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Type |
Journal
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Identifier |
Goon A., Shinde R., Ghosh B, Banerjee Kaushik (2019). Application of automated mini–Solid-Phase Extraction cleanup for the analysis of pesticides in complex spice matrixes by GC-MS/MS. Journal of AOAC International DOI: https://doi.org/10.5740/jaoacint.19-0202
https://doi.org/10.5740/jaoacint.19-0202 http://krishi.icar.gov.in/jspui/handle/123456789/38644 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Oxford University Press
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