Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by liquid chromatography with tandem mass spectrometry
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Title |
Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by liquid chromatography with tandem mass spectrometry
Not Available |
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Creator |
Oulkar D. P., Hingmire S., Goon A., Jadhav M., Ugare B., Shabeer A.T.P., Banerjee Kaushik
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Subject |
Glyphosate
Glufosinate LC-MS/MS Method of analysis Method validation |
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Description |
Not Available
A sensitive and accurate LC with tandem MS (MS/MS)-based method was developed and validated for the analysis of the herbicide glyphosate, its metabolite aminomethylphosphonic acid (AMPA), and glufosinate after derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) in various plant matrixes. The method also covers direct analysis of the glufosinate metabolites 3-methylphosphinicopropionic acid (3-MPPA) and N-acetyl-glufosinate (NAG). The homogenized samples were extracted with 0.1% formic acid in water–dichloromethane (50 + 50). The aqueous layer was derivatized with FMOC-Cl, cleaned through an HLB SPE cartridge, and determined by LC-MS/MS. The sample size, extraction solvent, sample-to-solvent ratio, derivatization conditions,and cleanup procedure were thoroughly optimized, the LOQs of glyphosate, glufosinate, and AMPA were 0.5 ng/g in grape, corn (leaf and seed), and cotton (leaf, seed, and oil) and 2 ng/g in soybean and tea. The LOQs of NAGand 3-MPPA were 50 ng/g in all the test matrixes, except tea and soybean, for which the LOQ was 100 ng/g. Inall cases, average recoveries were >80%. The method successfully performed the estimation of glyphosate in incurred corn and cotton leaf samples collected from supervised field trials. Not Available |
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Date |
2020-07-31T06:37:12Z
2020-07-31T06:37:12Z 2019-11-22 |
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Type |
Journal
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Identifier |
Oulkar D. P., Hingmire S., Goon A., Jadhav M., Ugare B., Shabeer A.T.P., Banerjee Kaushik (2017) Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by liquid chromatography with tandem mass spectrometry. Journal of AOAC International 100 (3): 631-639.
https://doi.org/10.5740/jaoacint.17-0046 http://krishi.icar.gov.in/jspui/handle/123456789/38666 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Oxford University Press
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