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Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by liquid chromatography with tandem mass spectrometry

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Title Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by liquid chromatography with tandem mass spectrometry
Not Available
 
Creator Oulkar D. P., Hingmire S., Goon A., Jadhav M., Ugare B., Shabeer A.T.P., Banerjee Kaushik
 
Subject Glyphosate
Glufosinate
LC-MS/MS
Method of analysis
Method validation
 
Description Not Available
A sensitive and accurate LC with tandem MS (MS/MS)-based method was developed and validated for the analysis of the herbicide glyphosate, its metabolite aminomethylphosphonic acid (AMPA), and glufosinate after derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) in various plant matrixes. The method also covers direct analysis of the glufosinate metabolites 3-methylphosphinicopropionic acid (3-MPPA) and N-acetyl-glufosinate (NAG). The homogenized samples were extracted with 0.1% formic acid in water–dichloromethane (50 + 50). The aqueous layer was derivatized with FMOC-Cl, cleaned through an HLB SPE cartridge, and determined by LC-MS/MS. The sample size, extraction solvent, sample-to-solvent ratio, derivatization conditions,and cleanup procedure were thoroughly optimized, the LOQs of glyphosate, glufosinate, and AMPA were 0.5 ng/g in grape, corn (leaf and seed), and cotton (leaf, seed, and oil) and 2 ng/g in soybean and tea. The LOQs of NAGand 3-MPPA were 50 ng/g in all the test matrixes, except tea and soybean, for which the LOQ was 100 ng/g. Inall cases, average recoveries were >80%. The method successfully performed the estimation of glyphosate in incurred corn and cotton leaf samples collected from supervised field trials.
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Date 2020-07-31T06:37:12Z
2020-07-31T06:37:12Z
2019-11-22
 
Type Journal
 
Identifier Oulkar D. P., Hingmire S., Goon A., Jadhav M., Ugare B., Shabeer A.T.P., Banerjee Kaushik (2017) Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by liquid chromatography with tandem mass spectrometry. Journal of AOAC International 100 (3): 631-639.
https://doi.org/10.5740/jaoacint.17-0046
http://krishi.icar.gov.in/jspui/handle/123456789/38666
 
Language English
 
Relation Not Available;
 
Publisher Oxford University Press