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Conservation of medicinal yam in vitro: Effect of ionic strength, sucrose, mannitol, ABA and low temperature,

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Title Conservation of medicinal yam in vitro: Effect of ionic strength, sucrose, mannitol, ABA and low temperature,
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Creator Not Available
 
Subject medicinal yam invitro conservation
 
Description Not Available
The effects of ionic strength, sucrose, ABA, mannitol and low temperature was investigated on the
development of slow growth cultures for in vitro conservation of medicinal yam germplasm. Though higher
concentration of mannitol (5 %), lower concentration of ionic strength (1/4 MS+ 0.5 % sucrose) and sucrose
(0.5 % and 1%) induced significantly high growth reduction in the plantlets, however, these treatments except
sucrose not only negatively affected survival of the cultures but also abnormal leaf morphology and multiple
shoot formation was observed with increase in concentration of mannitol. Sucrose (0.5 % and 1 %) not only
induced slow growth but also reduced the shoot production and showed no effect on survival of cultures even
maintained up to 180 days without subculturing. Storage of culture in normal MS media at low temperature
(18±1°C) maintained upto 150 days not only reduced the growth parameters but also induced development of
both basal and aerial microtubers. Use of low temperature could be the best option for monocrop repositories
of yam in vitro. However, reduced dose of sucrose can be a suitable and economically sustainable method for
in vitro conservation of D. floribunda at 25±2°C.
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Date 2021-01-12T11:35:10Z
2021-01-12T11:35:10Z
2019-12-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/44712
 
Language English
 
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Publisher Not Available