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Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants

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Title Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants
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Creator Snigdha Sameer Pattnaik , Byomkesh Dash, Sudhansu Sekhar Bhuyan , Jawahar Lal Katara , C. Parameswaran , Ramlakhan Verma , Narayanaperumal Ramesh and Sanghamitra Samantaray
 
Subject Androgenesis; doubled haploid; hybrid rice; ratoon; regeneration; spike
 
Description Not Available
An immense increase in human population along with diminished lands necessitates the
increase of rice production since, it serves the human population as a staple food. Though rice hybrids
(RH) are showing considerable yield enhancement over inbreds in terms of both quality and quantity,
farmers’ adoption of hybrid rice technology has been much slower than expected because of several
constraints such as seed cost and quality. Doubled haploid (DH) technology was considered useful
for the development of inbred lines from rice hybrids in a single generation. Androgenesis shows
its significance in development of DHs in rice which requires an efficient method to establish the
production of large population. To start the anther culture, anthers are the main component of
androgenesis to be isolated from unopened spikes. However, the duration of spikes availability for
anther culture coupled with the segregation of rice hybrids in the next generation requires the main
crop be ratooned to reduce the cost of cultivation. Therefore, the efficiency of the androgenic method
was tested in main crop using a quality indica rice hybrid, 27P63 and its ratooned ones. The effects of
various factors such as cold temperature pre-treatment of boots, treatment duration, and different
combination of plant growth regulators (PGR) on callus response along with shoot regeneration
were tested for development of DHs from both ratooned and non-ratooned plants. The N6 medium
supplemented with 2.0 mg/L 2,4-D (2,4-dichlrophenoxy acetic acid), 0.5 mg/L BAP (6-benzylamino
purine), and 30 g/L maltose was found to be most effective for callusing as compared to MS (Murashige
and Skoog) medium. The N6 media inducted calli showed maximum response rate for green shoot
regeneration in MS media supplemented with 0.5 mg/L NAA (1-napthaleneacetic acid), 0.5 mg/L
Kn (Kinetin; 6-furfurylaminopurine), 1.5 mg/L BAP and 30 g/L sucrose after 2 weeks of culture.
The pre-treatment of spikes at 10 ◦C for 2 d followed by a 7th and 8th d were found to be most effective
for callusing as well as for regeneration, producing a total of 343 green plants from ratooned and
main rice hybrid, 27P63. Morpho-agronomic trait-based assessment of ploidy status revealed 94.46%
diploids, 3.49% polyploids, 0.58% mixploids, and 1.45% haploids. Microsatellite markers could
authenticate all 324 fertile diploids as true DHs. Though this study shows a reduction in generation
of DHs from ratooned plants as compared to the main crop, manipulation of chemical factors could
optimize the method to enhance the production of considerable number of DHs. Utilization of
ratooned of hybrid rice in androgenesis would save time and cost of cultivation.
ICAR-NRRI, Cuttack
 
Date 2021-01-12T07:16:17Z
2021-01-12T07:16:17Z
2020-10-02
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/44690
 
Language English
 
Relation Not Available;
 
Publisher www.mdpi.com/journal/plants