Record Details

Overexpression of plasma membrane Na+/H+ antiporter OsSOS1 gene improves salt tolerance in transgenic rice plants.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Overexpression of plasma membrane Na+/H+ antiporter OsSOS1 gene improves salt tolerance in transgenic rice plants.
Not Available
 
Creator Awaji SM, Hanjagi PS, Pushpa BN and Sashidhar VR
 
Subject In planta, OsSOS1, Rice, Salt stress, transgenics
 
Description Not Available
Crop productivity is greatly affected by soil salinity; therefore, improvement in salinity tolerance of crops is a
major goal in salt-tolerant breeding. The Salt Overly Sensitive (SOS) signal-transduction pathway plays a key
role in ion homeostasis and salt tolerance in plants. In plants pumping of Na+ from the root cells is mediated by
the plasma membrane Na+/H+ antiporter (SOS1) which plays important role in preventing the accumulation of
toxic levels of Na+ in cytosol. In the present study, OsSOS1 (NHX7), gene was overexpressed in rice (var-Vikas)
by Agrobacterium mediated In Planta transformation technique. To screen putative T1 plants for salt tolerance,
stringent salt screening test was followed and root and shoot growth of transformants were used as
selection criterion. Some of the putative transgenics showed significantly higher root growth compared to wild
type. To confirm the presence of transgene in putative T1 transgenic plants, PCR based approach was followed
using genomic DNA. The result showed that 16 % of the selected seedlings from the stringent salt screening test
were PCR positives. Five selected lines were positive for RT-PCR analysis. Physiological studies such as
chlorophyll content, membrane permeability, cell viability and sodium /potassium content analysis were also
conducted to assess their levels of tolerance. Some of the T1 transformants showed lower percent reduction in
chlorophyll content and less membrane leakage, higher cell viability and maintained higher K/Na ratio after
NaCl treatment compared to wild type. These results clearly demonstrate that transgenic rice plants
overexpressing OsSOS1 have better salt-tolerance. This could be attributed to extrusion of excess Na+ from
cytosol into the apoplast and thereby reducing the toxic effects of Na+in the cell.
ICAR
 
Date 2021-01-12T07:03:14Z
2021-01-12T07:03:14Z
2020-12-31
 
Type Research Paper
 
Identifier Awaji SM, Hanjagi PS, Pushpa BN and Sashidhar VR (2020). Overexpression of plasma membrane Na+/H+ antiporter OsSOS1 gene improves salt tolerance in transgenic rice plants. Oryza, 57(4), 277-287. DOI https://doi.org/10.35709/ory.2020.57.4.3
0474-7615
http://krishi.icar.gov.in/jspui/handle/123456789/44684
 
Language English
 
Publisher Association of Rice Research Workers