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Rapid and sensitive detection of Xanthomonas axonopodis pv. punicae infecting pomegranate using loop-mediated isothermal amplification.

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Title Rapid and sensitive detection of Xanthomonas axonopodis pv. punicae infecting pomegranate using loop-mediated isothermal amplification.
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Creator T. R. Usharani, S. Meenakshi, C. Sunisha, H. D. Sowmya,. Shivshankar G Kumbdale, S. Poovarasan and S. Mohandas
 
Subject pomegranate LAMP Xanthomonas
 
Description Not Available
In the present study, Loop – mediated isothermal amplification (LAMP) assay was used as a novel tool fordiagnosis of Xanthomonas axonopodis pv. punicae (Xap), a gram negative host specific bacterium causingbacterial blight of pomegranate (Punica granatum). LAMP assay based on the amplification of the Gyrase â (gyrâ)gene showed the detection limit of 1fg/μl of purified Xap DNA when compared with conventional PCR showingamplification only upto 1pg/μl concentration of DNA. The time limit of detection was found to be 45 and 60 minof incubation. The sensitivity of this LAMP assay was determined to be 10 copies per reaction mixture as againstsingle copy detection using real time PCR.The LAMP assay was able to detect Xap from crude extract of theinfected samples collected from different locations without isolating the pathogen or purified genomic DNA.Pathogenic extract from infected leaf was visualized as increase in turbidity as well as color change from violet tosky blue by pre addition of HNB. LAMP assay showed a great potential for monitoring the disease incidence andit could prove to be a powerful supplemental tool for current diagnostic methods
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Date 2019-03-22T03:38:08Z
2019-03-22T03:38:08Z
2017-04-13
 
Type Article
 
Identifier Not Available
0973-7049
http://krishi.icar.gov.in/jspui/handle/123456789/17618
 
Language English
 
Relation Not Available;
 
Publisher NATIONAL ENVIRONMENTALISTS ASSOCIATION, INDIA.