Rapid and sensitive detection of Xanthomonas axonopodis pv. punicae infecting pomegranate using loop-mediated isothermal amplification.
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Title |
Rapid and sensitive detection of Xanthomonas axonopodis pv. punicae infecting pomegranate using loop-mediated isothermal amplification.
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Creator |
T. R. Usharani, S. Meenakshi, C. Sunisha, H. D. Sowmya,. Shivshankar G Kumbdale, S. Poovarasan and S. Mohandas
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Subject |
pomegranate LAMP Xanthomonas
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Description |
Not Available
In the present study, Loop – mediated isothermal amplification (LAMP) assay was used as a novel tool fordiagnosis of Xanthomonas axonopodis pv. punicae (Xap), a gram negative host specific bacterium causingbacterial blight of pomegranate (Punica granatum). LAMP assay based on the amplification of the Gyrase â (gyrâ)gene showed the detection limit of 1fg/μl of purified Xap DNA when compared with conventional PCR showingamplification only upto 1pg/μl concentration of DNA. The time limit of detection was found to be 45 and 60 minof incubation. The sensitivity of this LAMP assay was determined to be 10 copies per reaction mixture as againstsingle copy detection using real time PCR.The LAMP assay was able to detect Xap from crude extract of theinfected samples collected from different locations without isolating the pathogen or purified genomic DNA.Pathogenic extract from infected leaf was visualized as increase in turbidity as well as color change from violet tosky blue by pre addition of HNB. LAMP assay showed a great potential for monitoring the disease incidence andit could prove to be a powerful supplemental tool for current diagnostic methods Not Available |
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Date |
2019-03-22T03:38:08Z
2019-03-22T03:38:08Z 2017-04-13 |
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Type |
Article
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Identifier |
Not Available
0973-7049 http://krishi.icar.gov.in/jspui/handle/123456789/17618 |
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Language |
English
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Relation |
Not Available;
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Publisher |
NATIONAL ENVIRONMENTALISTS ASSOCIATION, INDIA.
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