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Detection and diagnosis of tomato leaf curl virus infecting tomato in Northern Karnataka

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Title Detection and diagnosis of tomato leaf curl virus infecting tomato in Northern Karnataka
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Creator Reddy, A. B., Patil, M. S., Reddy, M. K and Venkataravanappa
 
Subject ToLCV, detection, diagnosis, cloning, nucleotide sequence
 
Description Not Available
Tomato (Lycopersicon esculentum Mill.) is an important and most widely grown vegetable crop in India.
The begomo viruses’ affecting tomato in India is the most devastating and is a major limiting factor in
the tomato production. The tomato leaf curl virus (ToLCV) was present in almost all fields of Belgaum,
Dharwad and Haveri districts surveyed with the disease incidence ranged from 4-100% in rabi and was
in severe form ranging from 60-100% during summer. All the five representative symptomatic samples
collected from the different regions of North-Karnataka were found positive for polymerase chain
reaction (PCR) amplification with specific primers for deoxyribonucleic acid (DNA); a component of
tomato leaf curl Bangalore virus (ToLCBV). To examine the diversity of the sequences, phylogenetic
trees were generated for the four CP sequences together with representative sequences available in
gene Bank. The isolates under study clustered into two groups. The Dharwad isolate and Belgaum- 2
isolate were closely related (99.4% nucleotide similarity) and formed in to one cluster in which Haveri
and Belgaum-1 isolates had comparatively less homology (97.30% nucleotide homology) between
themselves and clustered into another sub group. The isolates under study had lowest nucleotide
sequence homology of 53.50 to 53.90% with ToLCV 19.Patna (AJ 810358) followed by ToLCV
18.Malvastrum.Pa (AJ 810357) (53.50-54.00%), ToLCV 17. Nasik (AJ 810356) (53.90-54.30%), while they
had highest homology of 92.40-96.00% with ToLCBV-AVT 1 (AY 428770). The results revealed that these
isolates are entirely different from North Indian isolates and there is some variability within the isolates
collected from a geographical location, indicating that there will be continuous variability in gemini
viruses.
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Date 2019-03-29T09:45:03Z
2019-03-29T09:45:03Z
2011-01-01
 
Type Article
 
Identifier 4
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http://krishi.icar.gov.in/jspui/handle/123456789/17797
 
Language English
 
Relation Not Available;
 
Publisher Not Available