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Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India, Plant

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Title Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India, Plant
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Creator Venkataravanappa, V., Swaranalatha, P., Lakshminarayana Reddy, C.N., Mahesh, B, Rai.A.B. and Krishna Reddy, M
 
Subject Common bean; Tobacco curly shoot virus (TbCSV); Tomato leaf curl betasatellite (ToLCB); PCR; Whitefly; Phylogentic analyses; Recombination
 
Description Not Available
A new strain (FB01) of Tobacco curly shoot virus (TbCSV) showing curly shoot symptoms on common bean
plants from Varanasi, Uttar Pradesh state of India was characterized. The analysis of the whole genome sequence
and individual ORFs of this virus indicated that it is very closely related (sequence similarity of 89.1-94.5%) to the
TbCSV infecting solanaceous and other weed crops in India and China. This was well supported by phylogenetic
analysis with close clustering of the virus isolate with TbCSV. The absence of DNA-B and association of virus with
betasatellite confirmed it as a monopartite begomovirus. The betasatellite identified here shared highest (53.9-
93.9%) sequence identity with tomato leaf curl betasatellite. Further, putative recombination events were recognized
within the virus sequence, suggesting that the virus is a recombinant and evolved from recombination of Tobacco
curly shoot virus, Munbean yellow mosaic virus, Tomato leaf curl Jodhpur virus, Tobacco leaf curl Yunnan virus
and Ageratum enation virus like ancestors. For betasatellite, the putative recombination events were recognized
within the sequence, were interspecific. The new recombinant betasatellite was derived from recombination between
Croton yellow vein mosaic betasatellite and Tomato yellow leaf curl China betasatellite, as the foremost parents in
its evolution. The virus was transmitted by whiteflies as well as sap, and not by seed.
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Date 2019-03-30T06:49:40Z
2019-03-30T06:49:40Z
2012-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17819
 
Language English
 
Relation Not Available;
 
Publisher Not Available