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Detection, Characterization and in-Silico Analysis of Candidatus Phytoplasma Australasia associated with Phyllody Disease of Sesame

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Title Detection, Characterization and in-Silico Analysis of Candidatus Phytoplasma Australasia associated with Phyllody Disease of Sesame
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Creator Venkataravanappa V, Reddy CNL, Manjunath M, Chauhan NS, Reddy MK
 
Subject Sesame Phyllody; PCR, Phytoplasma; 16SII group
 
Description Not Available
Leaf samples from sesame plants exhibiting Phyllody disease were collected from Varanasi and Mirzapur districts of Uttar Pradesh, India during the survey conducted between month of September to December, 2012-14. Incidence of sesame Phyllody in the farmers at different location was ranged from 30-70 percent indicating its prevalence in Uttar Pradesh. The Phytoplasma infection in sesame plants was confirmed by PCR using universal primers of 16s rRNA (R16F2n/R16R2) and SecY gene (SecYF2 and SecYR1) respectively. Amplified 16s rRNA and SecY gene was sequenced and sequence comparisons were made with the available Phytoplasma 16srRNA and SecY gene sequences in NCBI Gen Bank database. The 16srRNA and SecY gene sequence of Phytoplasma in the current study, shared highest nucleotide identity of 97.9 - 99.9% and 95.8 to 96.3% with subgroup 16Sr II-D the peanut witches’-broom group. A Comprehensive recombination analysis using RDP4 showed the evidence of inter- recombination in F2nR2 and SecY gene fragment of Phytoplasma infecting sesame. The most of the F2nR2 fragment is descended from Ash yellows-[16SrVIII] and Apple proliferation-[16SrX] group. While for SecY gene, most of the part was descended from Peanut witches’-broom- 16SrII-A (GU004331) and aster yellows 16Sr I-A (GU004345). The genetic similarities and the potential threat of this new Phytoplasma belong to 16Sr II-D subgroup of Peanut witches’ broom’ group infecting to sesame in north India are discussed.
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Date 2019-03-30T06:46:38Z
2019-03-30T06:46:38Z
2017-01-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/17816
 
Language English
 
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Publisher Not Available