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Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms

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Title Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms
Not Available
 
Creator Vineeth, R.
Sivaraman, G. K.
Ardhra Vijayan
Ravikrishnan Elangovan
Alison Prendiville
Bachmann, T. T.
 
Subject Not Available
 
Description Not Available
The population of methicillin-resistant (MR) staphylococci in aquatic environment is rarely investigated. Here, we characterized a collection of MR staphylococci recovered from shrimp aquaculture farms (n = 37) in Kerala, India. A total of 261 samples yielded 47 MR isolates (16 S. aureus, 13 S. haemolyticus, 11 S. epidermidis, 3 S. saprophytics and 2 each of S.intermedius and S. kloosii). Multi-drug resistance was evident in 72.3 % of the isolates, with resistance mainly towards erythromycin (78.7 % ), norfloxacin and trimethoprim-sulfamethoxazole (53.2 % ), and gentamicin (34 % ). Major resistance genes identified included mecA (100 % ), ermC (38.3 % ), aacA-aphD (21.3 % ), tetK (14.9 % ) and tetM (21.3
% ). Almost 60 % of the isolates carried type V SCCmec (Staphylococcal Cassette Chromosome mec), and the remaining harboured untypeable SCCmec elements. Comprehensive genotyping of the methicillin-resistant Staphylococcus aureus isolates revealed high prevalence of ST772-t345-V (sequence type-spa type-SCCmec type) (75 % ), followed by minor representations of ST6657-t345-V and ST3190-t12353. The isolates of S. haemolyticus and S. epidermidis were genotypically diverse as shown by their pulsed-field gel electrophoresis (PFGE) profiles. Genes encoding staphylococcal enterotoxins were observed in 53.2 % of the isolates. Various genes involved in adhesion and biofilm formation were also identified. In conclusion, our findings provide evidence that shrimp aquaculture settings can act as reservoirs of methicillin-resistant staphylococci.
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Date 2021-08-25T03:21:54Z
2021-08-25T03:21:54Z
2021-08-03
 
Type Research Paper
 
Identifier Vineeth, R., Sivaraman, G. K., Ardhra Vijayan, Ravikrishnan Elangovan, Alison Prendiville and Bachmann, T. T. (2021) Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms. Environ. Microbiol. Rep. https://doi.org/10.1111/1758-2229.12995
1758-2229
http://krishi.icar.gov.in/jspui/handle/123456789/60368
 
Language English
 
Relation Not Available;
 
Publisher Society for Applied Microbiology