Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms
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Title |
Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms
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Creator |
Vineeth, R.
Sivaraman, G. K. Ardhra Vijayan Ravikrishnan Elangovan Alison Prendiville Bachmann, T. T. |
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Subject |
Not Available
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Description |
Not Available
The population of methicillin-resistant (MR) staphylococci in aquatic environment is rarely investigated. Here, we characterized a collection of MR staphylococci recovered from shrimp aquaculture farms (n = 37) in Kerala, India. A total of 261 samples yielded 47 MR isolates (16 S. aureus, 13 S. haemolyticus, 11 S. epidermidis, 3 S. saprophytics and 2 each of S.intermedius and S. kloosii). Multi-drug resistance was evident in 72.3 % of the isolates, with resistance mainly towards erythromycin (78.7 % ), norfloxacin and trimethoprim-sulfamethoxazole (53.2 % ), and gentamicin (34 % ). Major resistance genes identified included mecA (100 % ), ermC (38.3 % ), aacA-aphD (21.3 % ), tetK (14.9 % ) and tetM (21.3 % ). Almost 60 % of the isolates carried type V SCCmec (Staphylococcal Cassette Chromosome mec), and the remaining harboured untypeable SCCmec elements. Comprehensive genotyping of the methicillin-resistant Staphylococcus aureus isolates revealed high prevalence of ST772-t345-V (sequence type-spa type-SCCmec type) (75 % ), followed by minor representations of ST6657-t345-V and ST3190-t12353. The isolates of S. haemolyticus and S. epidermidis were genotypically diverse as shown by their pulsed-field gel electrophoresis (PFGE) profiles. Genes encoding staphylococcal enterotoxins were observed in 53.2 % of the isolates. Various genes involved in adhesion and biofilm formation were also identified. In conclusion, our findings provide evidence that shrimp aquaculture settings can act as reservoirs of methicillin-resistant staphylococci. Not Available |
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Date |
2021-08-25T03:21:54Z
2021-08-25T03:21:54Z 2021-08-03 |
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Type |
Research Paper
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Identifier |
Vineeth, R., Sivaraman, G. K., Ardhra Vijayan, Ravikrishnan Elangovan, Alison Prendiville and Bachmann, T. T. (2021) Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms. Environ. Microbiol. Rep. https://doi.org/10.1111/1758-2229.12995
1758-2229 http://krishi.icar.gov.in/jspui/handle/123456789/60368 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Society for Applied Microbiology
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