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Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India

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Title Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India
Not Available
 
Creator Sivaraman, G. K.
Vineeth, R.
Ardhra Vijayan
Ravikrishnan Elangovan
Alison Prendiville
Bachmann, T. T.
 
Subject shrimp aquaculture
extended-spectrum beta-lactamase
Escherichia coli
Klebsiella pneumoniae
multidrug resistance
 
Description Not Available
This study was undertaken to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae in selected shrimp aquaculture farms (n = 37) in Kerala, South India and to characterize the isolates using molecular tools. Overall, a low prevalence of ESBL- producers was found in the farms, most likely due to the reduced antibiotic usage in the shrimp farming sector. Out of the 261 samples (77 shrimp and 92 each of water and sediment), 14 (5.4 % ) tested positive for ESBL-E. coli or ESBL-K. pneumoniae. A total of 32 ESBL-E. coli and 15 ESBL- K. pneumoniae were recovered from these samples. All ESBL isolates were cefotaxime-resistant with minimal inhibitory concentration (MIC) plus or minus 32 μg per ml. Of all isolates, 9 (28.1 % ) E. coli and 13 (86.7 % ) K. pneumoniae showed simultaneous resistance to tetracycline, ciprofloxacin, and trimethoprim-sulfamethoxazole. PCR analysis identified CTX-M group 1 (blaCTX–M–15) as the predominant ESBL genotype in both E. coli (23, 71.9 % ) and K. pneumoniae (15, 100 % ). Other beta-lactamase genes detected were as follows: blaTEM and blaSHV (11 K. pneumoniae), blaCTX – M group 9 (9 E. coli), and blaCMY–2 (2 E. coli). Further screening for AMR genes identified tetA and tetB (13, 40.6 % ), sul1 (11, 34.4%), sul2 (9, 28.1 % ), catA and cmlA (11, 34.4 % ), qepA and aac(6′)-Ib-cr (9, 28.1 % ) and strAB and aadA1 (2, 6.3%) in E. coli, and qnrB (13, 86.7%), qnrS (3, 20%), oqxB (13, 86.7%), tetA (13, 86.7 % ), and sul2 (13, 86.7 % ) in K. pneumoniae isolates. Phylogenetic groups identified among E. coli isolates included B1 (4, 12.5 % ), B2 (6, 18.8 % ), C (10, 31.3%), D (3, 9.4 % ), and E (9, 28.1 % ). PCR-based replicon typing (PBRT) showed the predominance of IncFIA and IncFIB plasmids in E. coli; however, in K. pneumoniae, the major replicon type detected was IncHI1. Invariably, all isolates of K. pneumoniae harbored virulence-associated genes viz., iutA, entB, and mrkD. Epidemiological typing by pulsed-field gel electrophoresis (PFGE) revealed that E. coli isolates recovered from different farms were genetically unrelated, whereas isolates of K. pneumoniae showed considerable genetic relatedness. In conclusion, our findings provide evidence that shrimp aquaculture environments can act as reservoirs of multi-drug resistant E. coli and K. pneumoniae.
Not Available
 
Date 2021-08-25T03:20:29Z
2021-08-25T03:20:29Z
2021-08-19
 
Type Research Paper
 
Identifier Sivaraman, G. K., Vineeth, R., Ardhra Vijayan, Ravikrishnan Elangovan, Alison Prendiville and Bachmann, T. T. (2021) Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India. Front. Microbiol. https://doi.org/10.3389/fmicb.2021.622891
1664-302X
http://krishi.icar.gov.in/jspui/handle/123456789/60367
 
Language English
 
Relation Not Available;
 
Publisher Frontiers Media S.A.