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Characterization of V. cholerae O1 biotype El Tor serotype Ogawa possessing the ctxB gene of the classical biotype isolated from well water associated with the cholera outbreak in Kerala, South India

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Title Characterization of V. cholerae O1 biotype El Tor serotype Ogawa possessing the ctxB gene of the classical biotype isolated from well water associated with the cholera outbreak in Kerala, South India
Not Available
 
Creator Reethy, P. S.
Lalitha, K. V.
 
Subject ERIC-PCR
multidrug resistance
PFGE
toxigenic strains
Vibrio cholerae
well water
 
Description Not Available
We investigated 22 water samples (17 well water and five pipe water – both chlorinated) and six soil samples from the surroundings of wells of the households of suspected patients from Palakkad district, Kerala (India), from where a cholera outbreak was reported during June – July 2016. A total of 25 Vibrio cholerae isolates were collected from three well water samples during a recent cholera outbreak. Biochemical and serological studies revealed that all of the isolates belonged to serogroup O1, biotype El Tor, serotype Ogawa. PCR assays confirmed the occurrence of ctxB, ctxA, hlyA, tcpA El Tor,VPI, ace, zot, ompW, rfbO1 and toxR genes in all isolates. The presence of the ctxB gene of the classical biotype in all of the El Tor isolates suggests that it is a new variant of El Tor biotype. Antibiogram profile of all V. cholerae O1 isolates revealed resistance towards five classes of antibiotics island and indicates that they were multidrug resistant. ERIC - PCR and PFGE finger prints showed the clonal relationship among the V. cholerae O1 isolates. The results of this study revealed the emergence of a new variant of El Tor biotype in the water samples from Palakkad district, from where a cholera outbreak was reported.
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Date 2021-09-18T05:00:15Z
2021-09-18T05:00:15Z
2021-04-05
 
Type Research Paper
 
Identifier Reethy, P. S. and Lalitha, K. V. (2021) Characterization of V. cholerae O1 biotype El Tor serotype Ogawa possessing the ctxB gene of the classical biotype isolated from well water associated with the cholera outbreak in Kerala, South India. J. Water Health 19 (3): 478–487.
1477-8920 (Print) 996-7829 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/64478
 
Language English
 
Relation Not Available;
 
Publisher IWA publishing