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Genotypic response of Stevia rebaudiana accessions to in vitro clonal propagation using nodal explants

Indian Agricultural Research Journals

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Title Genotypic response of Stevia rebaudiana accessions to in vitro clonal propagation using nodal explants
 
Creator SINGH, SANATSUJAT
YADAV, ASHOK KUMAR
 
Subject Genotypic response, In vitro clonal propagation, Nodal explant, Stevia rebaudiana
 
Description Genotypic response to in vitro clonal propagation was studied in 21 accessions of Stevia rebaudiana. Data were recorded with respect to per cent proliferation, root induction and survival during hardening process and Chi-square test for goodness of fit was used to determine the variations regarding response to different nutrient media used for in vitro clonal propagation. Nodal explants of Stevia rebaudiana can regenerate shoots when cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyl amino purine (BAP; 1 mg/l) and kinetin (Kn; 0.5 mg/l). Rooting of in vitro shoots was achieved by adding indole butyric acid (IBA; 0.2mg/l) on half strength MS medium. The accessions C1-4-2,E2-1-6, C1-4-3, E1-2-3 and C1-4-3 of stevia were identified as responsive genotypes to in vitro clonal propagation and have potential use in clonal breeding programmes.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2013-05-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/30360
 
Source The Indian Journal of Agricultural Sciences; Vol 83, No 6 (2013)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/30360/13660
 
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