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Molecular characterization of Alternaria carthami of safflower using RAPD and ISSR markers.

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Title Molecular characterization of Alternaria carthami of safflower using RAPD and ISSR markers.
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Creator Naresh, N.
Santha Lakshmi Prasad, M.
Sujatha, K.
 
Subject Alternaria carthami
Genetic similarity
ISSR
RAPD
 
Description Not Available
In the present study, the intra and inter specific polymorphisms among the isolates of Alternaria earthami
which causes leaf blight on Safflower (Carthamus tinctorius L.) were investigated by employing 20 Random
Amplified Polymorphic DNA (RAPD) and 15 Inter Simple Sequence Repeats (ISSR) markers. The genetic
surulanty was studied by using Unwilghted Pair Group Mean Average (UPGMA) cluster analysis of RAPD and
ISSR datasets by employing NTSYSPc (V2.0). Similarity co-efficient differentiated all isolates from each other and revealed considerable molecular variability between the isolates. Among the five isolates, the percentage of polymorphism was observed was 65% with RAPD and 50.6% with ISSR primers. The results show that RAPD and ISSR markers were suitable for the evaluation of genetic polymorphisms among the isolates of A. carthami.
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Date 2021-09-20T04:00:25Z
2021-09-20T04:00:25Z
2012
 
Type Research Paper
 
Identifier Naresh, N., Santha Lakshmi Prasad, M. and Sujatha, K. 2012. Molecular characterization of Alternaria carthami of safflower using RAPD and ISSR markers. Journal of Oilseeds Research, 29 (Spl. Issue): 336-338.
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http://krishi.icar.gov.in/jspui/handle/123456789/64532
 
Language English
 
Relation Not Available;
 
Publisher Not Available