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Identification of Promising RILs for High Grain Zinc through Genotype x Environment Analysis and Stable Grain Zinc QTL using SSRs and SNPs in rice (Oryza sativa L.).

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Title Identification of Promising RILs for High Grain Zinc through Genotype x Environment Analysis and Stable Grain Zinc QTL using SSRs and SNPs in rice (Oryza sativa L.).
Not Available
 
Creator K Suman, C N Neeraja, P Madhubabu, Santosha Rathod, Sonali Bej, K P Jadhav, J Aravind Kumar J, U Chaitanya, Smita C Pawar, K Surekha, H Rani, L V Subbarao, S R Voleti
 
Subject Rice, grain zinc, RILs, stability, QTL, SSR, SNP
 
Description Not Available
Polished rice is one of the commonly consumed staple foods across the world. However,
it contains limited nutrients especially iron (Fe) and zinc (Zn). To identify promising
recombinant inbred lines (RILs) for grain Zn and single plant yield, 190 RILs developed
from PR116 and Ranbir Basmati were evaluated in two environments (E1 and E2).
A subset of 44 contrasting RILs for grain Zn was screened in another two environments
(E3 and E4). Phenotypic data was collected for 10 traits, viz., days to 50% flowering,
plant height, panicle length, number of tillers, single plant yield (SPY), test weight, Fe
and Zn in brown (IBR, ZBR), and polished rice (IPR, ZPR). Stepwise regression analysis
of trait data in 190 RILs and a subset of 44 RILs revealed the interdependence of ZPR,
ZBR, IPR, and IBR and the negative association of grain Zn with single plant yield.
Based on the additive main effect and multiplicative interaction (AMMI) and genotype
and genotype × environment interaction (GGE) analyses of the subset of 44 RILs across
four environments (E1–E4), six promising RILs were identified for ZPR with >28 ppm.
Mapping of 190 RILs with 102 simple sequence repeats (SSRs) resulted in 13 QTLs
for best linear unbiased estimates (BLUEs) of traits including advantage over check
(AOC). Using genotype-based sequencing (GBS), the subset of 44 RILs was mapped
with 1035 single-nucleotide polymorphisms (SNPs) and 21 QTLs were identified. More
than 100 epistatic interactions were observed. A major QTL qZPR.1.1 (PV 37.84%)
and another QTL qZPR.11.1 (PV 15.47%) were identified for grain Zn in polished rice.
A common major QTL (qZBR.2.1 and qZPR.2.1) was also identified on chromosome 2
for grain Zn content across SSR and SNP maps. Two potential candidate genes related
to transporters were identified based on network analyses in the genomic regions of
QTL < 3 Mb. The RILs identified for grain Zn and SPY were nominated for national
evaluation as under rice biofortification, and two QTLs identified based on BLUEs could
be used in the rice biofortification breeding program
ICAR CRP Biofortification
 
Date 2021-08-17T06:06:49Z
2021-08-17T06:06:49Z
2021-02-18
 
Type Research Paper
 
Identifier Suman K, Neeraja C N, Madhubabu P, Santosha Rathod, Sonali Bej, Jadhav K P Aravind Kumar J, Chaitanya U , Smita C Pawar, Surekha H Rani, Subbarao L V, Voleti S R. (2021). Identification of Promising RILs for High Grain Zinc through Genotype x Environment Analysis and Stable Grain Zinc QTL using SSRs and SNPs in rice (Oryza sativa L.). Front. Plant Sci. | doi: 10.3389/fpls.2021.587482
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/57459
 
Language English
 
Relation Not Available;
 
Publisher Frontiers