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Construction and screening of metagenomic library derived from soil for β-1, 4-endoglucanase gene

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Title Construction and screening of metagenomic library derived from soil for β-1, 4-endoglucanase gene
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Creator Pandey, S. Gulati, S., Goyal, E., Singh, S., Kumar, K., Nain, L. and Saxena, A.K
 
Subject Metagenomic libraryCellulaseFunction based screeningβ-1, 4-endoglucanasePaper and pulp mill
 
Description Not Available
A metagenomic library consisting of 7500 clones was constructed from the soil continuously irrigated with effluents of paper and pulp mill. A cellulase positive clone H-10 was identified by function based screening. A β-1, 4- endoglucanase gene with an open reading frame of 1499 bp was identified from this positive clone. The deduced amino acid sequence analysis revealed that amplified β-1, 4-endoglucanase belongs to GH5 and shares 98% similarity with Bacillus subtilis (EF070194), 95% with Bacillus subtilis IARI-SP-1(KF240847) 96% with B. subtilis (AY183475) and 95% with uncultured bacterium clone (JX567739). Clone H-10 indicated higher CMCase activity of 1 IU/ml on the substrate CMC as compared to rice straw (0.5 IU/ml). The characterization of enzymes revealed its pH optima between 8.5–9 and retained 80% of activity at pH 11. The thermal profile of the enzyme indicated broad range of thermotolerance.
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Date 2021-09-25T16:06:07Z
2021-09-25T16:06:07Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/64689
 
Language English
 
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Publisher Not Available