Construction and screening of metagenomic library derived from soil for β-1, 4-endoglucanase gene
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Title |
Construction and screening of metagenomic library derived from soil for β-1, 4-endoglucanase gene
Not Available |
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Creator |
Pandey, S. Gulati, S., Goyal, E., Singh, S., Kumar, K., Nain, L. and Saxena, A.K
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Subject |
Metagenomic libraryCellulaseFunction based screeningβ-1, 4-endoglucanasePaper and pulp mill
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Description |
Not Available
A metagenomic library consisting of 7500 clones was constructed from the soil continuously irrigated with effluents of paper and pulp mill. A cellulase positive clone H-10 was identified by function based screening. A β-1, 4- endoglucanase gene with an open reading frame of 1499 bp was identified from this positive clone. The deduced amino acid sequence analysis revealed that amplified β-1, 4-endoglucanase belongs to GH5 and shares 98% similarity with Bacillus subtilis (EF070194), 95% with Bacillus subtilis IARI-SP-1(KF240847) 96% with B. subtilis (AY183475) and 95% with uncultured bacterium clone (JX567739). Clone H-10 indicated higher CMCase activity of 1 IU/ml on the substrate CMC as compared to rice straw (0.5 IU/ml). The characterization of enzymes revealed its pH optima between 8.5–9 and retained 80% of activity at pH 11. The thermal profile of the enzyme indicated broad range of thermotolerance. Not Available |
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Date |
2021-09-25T16:06:07Z
2021-09-25T16:06:07Z 2016-01-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/64689 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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