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Characterization of 14-3-3 like protein gene from C. falcatum challenged sugarcane, using RACE-PCR

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Title Characterization of 14-3-3 like protein gene from C. falcatum challenged sugarcane, using RACE-PCR
Not Available
 
Creator Rahul, P. R., V. Ganesh Kumar, R. Viswanathan, A. Ramesh Sundar and P. Malathi
 
Subject 14-3-3 like protein, RNA ligase, dendrogram , sugarcane disease resistance mechanism
 
Description Not Available
The present study reports the full length sequencing of 14-3-3 like protein transcript from differential display (DD)-RT-PCR. The gene was characterized using the RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE-PCR) technique. A 1094 bp, 14-3-3 like protein transcript (Genbank Acc. no. HO222097) revealed the presence of conserved 14-3-3 superfamily domain in the CDD search. The ORF finder tool from the NCBI, revealed the presence of a 771 bp ORF, flanked by 86 bp 5'-UTR, and 237 bp 3'-UTR in the full length sequence. The gene product was 256 amino acids in length and the calculated molecular weight was found to be 28.8 kDa. The studies involving host-pathogen interaction, wherein the 14-3-3 plays significant role is by regulating target proteins with functions of either signalling or transcription activation or defense. The multiple sequence alignment and their dendrogram revealed that the isolated 14-3-3 was more similar to rice GF14C followed by sugarcane cluster SCBFLR1026E02.g from SUCESTdatabase. Thus the identification of 14-3-3 during the incompatible interaction between sugarcane - C. falcatum is consistent with the expression pattern of defence-related genes during the host-pathogen interactions. An earlier report had suggested that 14-3-3 proteins, together with a SnRK1 (SNF-like kinases), phosphorylate and inhibit the enzyme sucrose phosphate synthase (SPS) in vitro in sugarcane. Thus the reduced expression of 14-3-3 in mature internodes may suggest an increased accumulation of sucrose content. Thus the interplay between different components of protein regulatory mechanism suggests a complex modulatory role for different isoforms of 14-3-3s thus playing an important role in response to a specific stimulus. The present study on 14-3-3 like protein indicated a specific induction upon interaction with C. falcatum, which suggest an implication for 14-3-3 to play an important role in sugarcane disease resistance mechanism. The information on the different isoforms of the 14-3-3 like protein in sugarcane is unavailable and future studies towards greater understanding of its role and their interplay needs to be established as in other crops.
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Date 2021-08-26T06:06:00Z
2021-08-26T06:06:00Z
2012-10-15
 
Type Presentation
 
Identifier Not Available
Not Available
International Symposium on New Paradigms in Sugarcane Research
http://krishi.icar.gov.in/jspui/handle/123456789/60609
 
Language English
 
Relation Not Available;
 
Publisher Society for Sugarcane Research and Development, Coimbatore, India