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WRKY transcription factor in sugarcane defence against Colletotrichum falcatum

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Title WRKY transcription factor in sugarcane defence against Colletotrichum falcatum
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Creator Nandakumar M., R. Viswanathan, P. Malathi and A. Ramesh Sundar
 
Subject sugarcane, red rot, Colletotrichum falcatum, WRKY TF’s, ScWRKY, FastPCR
 
Description Not Available
In sugarcane, red rot is a major disease caused by Colletotrichum falcatum Went which infects cane stalks. The infected stalks become unfit for milling and quality of juice is affected due to inversion of glucose and fructose. The function of WRKY TF’s in defense is proven as several EST-contigs were identified. Also a ScWRKY gene was identified for its active role in defence response against Sporisorium scitamineum, salicylic acid, drought and salinity. Among five different transcription factors studied in sugarcane during C. falcatum interaction, WRKY showed differential expression pattern in resistant and susceptible varieties.In continuation of the previous studies, we have taken up detailed studies to identify active involvement of WRKY TF’s during sugarcane and C. falcatum interaction. A total of 36 ScWRKY sequences were retrieved and primers were designed using FastPCR tool (Ver. 3.6.62). Expression analyses were performed with the sugarcane cultivars Co 93009(R) and CoC 671(S) which were inoculated with C. falcatum pathotype Cf671 conidial suspension (1x106/mL-1) and the samples were collected at 0, 6, 12, 24, 36, 48 and 72h later. RNA was isolated from the samples and expression studies were taken up by RT-PCR. Among the 36 ScWRKY primers, 16, 18, 19, 26, 31 and 35 primers showed expression and they were further used to study the spatial and temporal expression pattern during C. falcatum interaction with sugarcane. WRKY 16, 18 and19 showed higher expression in susceptible cultivar at 48h where as it was expressed low in other intervals. However the resistant cultivar showed early and late induction. WRKY 26 expressed in early stages in both susceptible and resistant samples. Also WRKY 31 and 35 showed high expression in the susceptible cultivar compared to the resistant cultivar. Further studies are being continued to identify specific expression of resistance associated transcription factor in WRKY family and their relation to expression of genes involved in defence related signal transduction pathway
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Date 2021-08-28T10:13:28Z
2021-08-28T10:13:28Z
2014-06-25
 
Type Proceedings
 
Identifier Not Available
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Symposium on Bioenergy for Sustainable Development- The Potential Role of Sugar Crops,
http://krishi.icar.gov.in/jspui/handle/123456789/60939
 
Language English
 
Relation Not Available;
 
Publisher Society for Sugarcane Research and Development, Coimbatore, India