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Ab initio prediction of micro-RNA like structures in sugarcane viruses and their cellular targets.

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Title Ab initio prediction of micro-RNA like structures in sugarcane viruses and their cellular targets.
Not Available
 
Creator Brindha S. and R. Viswanathan
 
Subject Sugarcane yellow leaf virus (SCYLV), Sugarcane streak mosaic virus (SCSMV), Sugarcane bacilliform virus (SCBV) ,
 
Description Not Available
During a viral infection in a plant, miRNAs interplay between the host and the pathogen. Sugarcane yellow leaf virus (SCYLV), Sugarcane streak mosaic virus (SCSMV), and Sugarcane bacilliform virus (SCBV) are serious diseases affecting sugarcane productivity worldwide. As a viable strategy to utilize resistance to the viruses in sugarcane, we used computational approaches to predict virus-encoded miRNAs in these viruses. We used an in silico approach to analyse SCYLV, SCSMV and SCBV virus genome sequences. Pre-miRNAs were extracted through VMir software, and screened using web tools such as Mir Para, MiPred, Mature Pred and Mature Bayes. The potential target genes were predicted using psRNATarget. From computational tools, different viral pre-miRNA hairpin sequences and 11, 8 and 13 putative mature miRNAs were predicted from SCYLV, SCSMV and SCBV genomes, respectively. These predicted miRNAs have hybridized with numerous targets. The present study is the first computational prediction of SCYLV and SCBV encoded viral miRNA and their targets in sugarcane and other monocot ESTs. This is also the first study that has identified miRNAs in P0 (SCYLV)-ORF, which is suppressor of RNA silencing. This new findings will fine-tune our strategy of using pathogen-derived resistance in sugarcane and developing virus-resistant varieties through conventional breeding
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Date 2021-08-28T09:57:35Z
2021-08-28T09:57:35Z
2016-01-01
 
Type Proceedings
 
Identifier Not Available
Not Available
Proceedings of the International Society of Sugar Cane Technologists,
http://krishi.icar.gov.in/jspui/handle/123456789/60918
 
Language English
 
Relation Not Available;
 
Publisher International Society of Sugar Cane Technologists