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Transcriptome analysis of host-pathogen interaction between sugarcane and Colletotrichum falcatum by Suppression Subtractive Hybridization and Illumina sequencing

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Title Transcriptome analysis of host-pathogen interaction between sugarcane and Colletotrichum falcatum by Suppression Subtractive Hybridization and Illumina sequencing
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Creator Viswanathan, R., M Sathyabhama, P Malathi and A. Ramesh Sundar
 
Subject Sugarcane, Colletotrichum falcatum, Illumina sequencing, resistance and susceptible responses, KEGG-KASS, PRR, signaling pathways, qRT-PCR
 
Description Not Available
A high throughput analyses of interaction between sugarcane and Colletotrichum falcatum, the hemibiotrophic pathogen causing red rot, was made by comparing transcriptomes of compatible and incompatible interactions in a tropical sugarcane cultivar Co7805 exhibiting differential reaction to the pathotypes Cf94012 and Cf87012, respectively. Suppression subtractive hybridization (SSH) combined with Illumina 2000 high throughput sequencing was used to identify the differential transcripts in resistance response library (RRL) and susceptible response library (SRL). After processing and filtering the raw reads from Illumina 2000 high throughput sequencing of subtracted products, we obtained 10,038 and 4,022 high quality transcripts, from RRL and SRL, respectively. Based on the transcripts mapping to KEGG-KASS database, the presence of a CEBiP receptor and the signals ROS, Ca2+, BR, JA and ABA were identified in both the responses. However, MAPK, ET, PI signals and JA amino conjugation were found only in the incompatible interaction and expression of 10 transcripts involved in these pathways was validated using qRT-PCR. Our study concludes that perception of PAMPs occurs in both systems, but downstream signaling through MAPK, ET, PI and JA amino conjugation and activation of R genes occurs only in the incompatible interaction. This is the first detailed transcriptomic analysis of compatible and incompatible interactions in sugarcane with two different C. falcatum pathotypes through SSH and the next generation sequencing (NGS) platform.
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Date 2021-09-02T05:33:27Z
2021-09-02T05:33:27Z
2016-01-01
 
Type Proceedings
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/61196
 
Language English
 
Relation Not Available;
 
Publisher International Society of Sugar Cane Technologists