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Characterization of ORF0 and ORF1 and their Roles in Recombination and Replication of Sugarcane yellow leaf virus

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Title Characterization of ORF0 and ORF1 and their Roles in Recombination and Replication of Sugarcane yellow leaf virus
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Creator ElSayed A.I., M. Boulila, D.C. Odero, A.A. Omar, A.M. Gomaa and R. Viswanathan
 
Subject Open reading frame, phylogenetic analysis, recombination, Sugarcane yellow leaf virus, qRT-PCR
 
Description Not Available
Three open reading frames (ORF0, ORF1 and ORF3) of Sugarcane yellow leaf virus (SCYLV) genome which play an important role in virus replication and accumulation in plants have been characterized. Transcript levels of three genes varied among infected plants but overall expression of ORF1 was higher than ORF0 and ORF3. Cultivar H73-6110 (susceptible) yielded the highest transcript levels of ORF1 whereas cultivar H78-4153 (resistant) exhibited the lowest levels. The sugarcane cultivars exhibited no significant differences between them for the ORF0 transcripts in mature leaves and seedling tissues. The ORF0, ORF1 and ORF3 of the SCYLV isolates varied in amino acid sequence similarity and it was in the ranges of 69-99%, 73-99%, and 93-100%, respectively. Possible recombination events located in the three ORFs were identified using TOPALi (v2.5), RECCO, and RDP software’s. The results showed strong presence of recombination in the aligned sequences of ORF0 and ORF1 when TOPALi and RECCO programs were used. In contrast, no recombination signals were detected in ORF3 with all the analysis tools used in this study. Similarly, the RDP program did not reveal any recombination signals in ORF0 and ORF3 with the exception of ORF1 where numerous accessions were found to be recombinants
Not Available
 
Date 2021-09-02T05:32:57Z
2021-09-02T05:32:57Z
2016-12-15
 
Type Research Paper
 
Identifier Not Available
2150-4210
http://krishi.icar.gov.in/jspui/handle/123456789/61195
 
Language English
 
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Publisher Not Available