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Studies on expression pattern of toll-like receptor 5 (TLR5) in Edwardsiella tarda infected Pangasianodon hypophthalmus.

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Title Studies on expression pattern of toll-like receptor 5 (TLR5) in Edwardsiella tarda infected Pangasianodon hypophthalmus.
 
Creator Poojashree Kachigere Jayaramu
Gayatri Tripathi
Annam Pavan Kumar
Jeena K
Mujahidkhan Ajamalkhan Pathan
K. Pani Prasad
 
Subject Toll like receptor,Innate immunity,E. tarda,mRNA expression,Real time quantitative PCR
 
Description Not Available
TLR5 is one of the important PRR (pathogen recognition receptors) and plays a fundamental role in pathogen recognition and activation of innate immune responses. It recognizes bacterial flagellin and stimulates the production of proinflammatory cytokines, through signalling via the adaptor protein MyD88. In this study, we characterized partial TLR5 (soluble form) gene from Pangasianodon hypophthalmus and analysed its expression profile upon challenge by Edwardsiella tarda. Bioinformatic analysis of gene sequence revealed a putative protein of 266 amino acids with four Leucine rich repeats. Quantitative expression analysis of TLR 5S showed its wide distribution in various organs and tissues. However, significant expression of TLR5S was observed in liver and spleen at 12 h (207.8 fold, p < 0.05). Significant upregulation was observed in kidney at 72 h.p.i. (50 folds, p < 0.05) indicating that the kidney provides longer protection almost till the activation of the adaptive immune system. This study enriches the knowledge of TLR5S in boosting the innate immunity against bacterial invasion in fish.
 
Date 2021-11-03T06:48:36Z
2021-11-03T06:48:36Z
2017-1-31
 
Type Research Paper
 
Identifier Jayaramu, P.K., Tripathi, G., Kumar, A.P., Keezhedath, J., Pathan, M.K. and Kurcheti, P.P., 2017. Studies on expression pattern of toll-like receptor 5 (TLR5) in Edwardsiella tarda infected Pangasianodon hypophthalmus. Fish & shellfish immunology, Vol. & Issue: 63(1), pp.68-73
Not Available
10504648
http://krishi.icar.gov.in/jspui/handle/123456789/67017
 
Language English
 
Publisher Not Available