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Antibacterial and antioomycete activities of a novel designed RY12WY peptide against fish pathogens.

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Title Antibacterial and antioomycete activities of a novel designed RY12WY peptide against fish pathogens.
 
Creator Raja Aadil Hussain Bhat
Dimpal Thakuria
Vinita Pant
Victoria C. Khangembam
Ritesh Shantilal Tandel
Neetu Shahi
Debajit Sarma
Gayatri Tripathi
Kishore Kumar Krishnani
Gopal Krishna
 
Subject Antibiotics,Antimicrobial peptides,Bacteria,Antimicrobial activity,Molecular docking
 
Description Not Available
In the present study, we have designed and synthesized a short compositionally simple peptide RY12WY having potent antimicrobial activity. The molecular docking study results showed that peptide has a strong affinity towards two protein targets of A. sobria; aerolysin and outer membrane protein (OMP). The MIC values ranged from 0.98 to 500 ?M and MBC values ranged from 4 to 650 ?M against the selected bacterial and fungal pathogens. The intense antimicrobial activity of RY12WY is reported against A. sobria, A. hydrophila, E. tarda, S. aureus, V. parahaemolyticus, P. aeruginosa and E.coli at low concentration.The peptide also showed good activity against A. salmonicida and S. parasitica zoospores. The peptide retained its antimicrobial activity at higher temperatures. Besides, it was active in the presence of physiological salts and serum.The peptide showed negligible haemolytic activity at 125 ?M and HC50 was found to be 1437.10 ?M. The DNA binding assay indicated that peptide can bind with the genetic material of the bacteria and may inhibit its replication. The bacterial viability assay reported that the peptide interferes with bacterial membrane integrity. To conclude, the results suggest that RY12WY could be a promising therapeutic agent in aquaculture and has possible application in food processing industry which warrants higher temperatures.
 
Date 2021-11-08T08:09:16Z
2021-11-08T08:09:16Z
2020-10-23
 
Type Research Paper
 
Identifier Bhat, R.A.H., Thakuria, D., Pant, V., Khangembam, V.C., Tandel, R.S., Shahi, N., Sarma, D., Tripathi, G., Krishnani, K.K. and Krishna, G., 2020. Antibacterial and antioomycete activities of a novel designed RY12WY peptide against fish pathogens. Microbial Pathogenesis, Vol. & Issue: 149(1), pp: 1045
Not Available
0882-4010
http://krishi.icar.gov.in/jspui/handle/123456789/67132
 
Language English
 
Publisher Not Available