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Development of a Cell Culture System From Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider)

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Title Development of a Cell Culture System From Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider)
Not Available
 
Creator Sobhana,K S
George,K C
Venkat Ravi,G
Ittoop,Gijo
Paulraj,R
 
Subject Cell Culture System
Gill Explants
Grouper
Epinephelus malabaricus
 
Description Not Available
A cell culture system was developed successfully from gill explants of the Malabar grouper,
Epinephelus malabaricus. Gill tissue samples aseptically excised from healthy juveniles of
E. malabaricus were explanted in Leibovitz’ L-15 medium supplemented with 0.07 M NaCl and
20% fetal bovine serum (FBS). A mixture of different types of cells emerged from the explants,
and these cells were observed to spread and attach to the culture flasks from the second day
onwards. Confluent monolayers comprising epithelioid as well as fibroblast-like cells were formed
within ten days. The cells were found to grow well at 28 + 2°C. The cell monolayers were
subcultured by trypsinization and seeded into new flasks, which produced confluent monolayers
comprising predominantly epithelioid-like cells in subsequent passages.
Not Available
 
Date 2021-09-15T09:26:01Z
2021-09-15T09:26:01Z
2009
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/62404
 
Language English
 
Relation Not Available
 
Publisher Asian Fisheries Soceity; Malaysia