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Effect of extender composition on sperm cryopreservation of Asian catfish Heteropneustes fossilis (Bloch) and Clarias batrachus (Linnaeus)

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Title Effect of extender composition on sperm
cryopreservation of Asian catfish Heteropneustes
fossilis (Bloch) and Clarias batrachus (Linnaeus)
Not Available
 
Creator Lakra,W S
Sah,Rama S
Gopalakrishnan,A
Lal,Bechan
Mohindra,Vindhya
Khare,Praveen
Punia,P
Lal,Kuldeep Kumar
Barman,A S
 
Subject sperm cryopreservation
Asian catfish
Heteropneustes
fossilis
Clarias batrachus
 
Description Not Available
Air breathing catfish species Heteropneustes fossilis (Bloch) and Clarias batrachus
(Linn.) are important table fish and fetch high market price. Cryopreservation of spermatozoa
can be a useful tool in captive seed production for domestication and aquaculture of these catfish
species. The objective of the present study was to identify optimum extender composition for
sperm cryopreservation of the two species, H. fossilis and C. batrachus. Four extender
compositions Hank’s Balanced Salt Solution (HBSS), Modified Hank’s Balanced Salt Solution
(M-HBSS), Modified Hank’s Balanced Salt Solution with hen’s egg yolk (M-HBSS with EY)
and European catfish were evaluated for cryopreservation of catfish sperm and 10 % Dimethyle
Sulphoxide (DMSO) was used as a cryoprotectant. The pooled milt exhibiting 70-80% motile
sperm was used for cryopreservation experiment. After storage for 48 hrs at -196ºC, the milt was
thawed and evaluated for fertility test. The percentage of hatching was used as a parameter for
the comparative evaluation of different extender composition. In H. fossilis extender M-HBSS
indicated highest hatching rate (49.06%), followed by HBSS (42.76%), M-HBSS with EY
(37.46%) and European catfish (29.47%). The hatching success with extender M-HBSS did not
differ significantly (P > 0.05) from the control group (51%). In C. batrachus extender HBSS
exhibited highest hatching (62.1 %), followed by M-HBSS with EY (51.6%), European Catfish
(46.3%) and M-HBSS (40.9%). The hatching rate in control was 90% in C. batrachus. The
results indicated that the two species differ in the protocol for sperm cryopreservation. The
paper presents successful cryopreservation of sperm with the production of viable hatchlings of
H. fossilis and C. batrachus for the first time. The protocol reported in the study can be used for
scaling up of seed production of these two catfish species.
Not Available
 
Date 2021-09-15T09:26:02Z
2021-09-15T09:26:02Z
2009
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/62413
 
Language English
 
Relation Not Available
 
Publisher AFS Society