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Genetic variation and population structure of endemic yellow catfish, Horabagrus brachysoma (Bagridae) among three populations of Western Ghat region using RAPD and microsatellite markers

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Title Genetic variation and population structure of endemic yellow
catfish, Horabagrus brachysoma (Bagridae) among three
populations of Western Ghat region using RAPD
and microsatellite markers
Not Available
 
Creator Basheer,V S
Lal,Kuldeep Kumar
Mohindra,Vindhya
Musammilu,K K
Gopalakrishnan,A
Abdul Muneer,P M
Lakra,W S
 
Subject Horabagrus brachysoma
RAPD
Genetic variation
Microsatellites
PCR
Polymorphism
 
Description Not Available
Random amplified polymorphic DNA (RAPD)
and microsatellite markers were applied to evaluate the
genetic variation in endemic and endangered yellow catfish,
Horabagrus brachysoma sampled from three geographic
locations of Western Ghat, South India river systems.
In RAPD, of 32 10-mer RAPD primers screened initially,
10 were chosen and used in a comparative analysis of
H. brachysoma collected from Meenachil, Chalakkudy and
Nethravathi River systems. Of the 124 total RAPD fragments
amplified, 49 (39.51%) were found to be shared by
individuals of all 3 populations. The remaining 75 fragments
were found to be polymorphic (60.48%). In microsatellites,
six polymorphic microsatellite loci were identified by using
primers developed for Pangasius hypophthalmus, Clarias
macrocephalus and Clarias gariepinus. The identified loci
were confirmed as microsatellite by sequencing after making
a clone. The nucleotide sequences of 6 loci were
published in NCBI genbank. The number of alleles across
the six loci ranged from 4 to 7 and heterozygosities ranged
from 0.07 to 0.93. The mean number of alleles and effective
number of alleles per locus were 5.00 and 3.314, respectively.
The average heterozygosity across all investigated
samples was 0.72, indicating a significant deficiency of
heterozygotes in this species. RAPD and microsatellite
methods reported a high degree of gene diversity and genetic
distances depicted by UPGMA dendrograms among the
populations of H. brachysoma.
Not Available
 
Date 2021-09-15T09:54:16Z
2021-09-15T09:54:16Z
2009
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/62434
 
Language English
 
Relation Not Available
 
Publisher Kluwer