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Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses?

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Title Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses?
Not Available
 
Creator Sukumaran,Sandhya
Grant,Alastair
 
Subject Artemia franciscana
Artemia parthenogenetica
Ethyl methanesulfonate
Genotoxicity
Comet assay
Fitness effects
 
Description Not Available
The impactofchronicgenotoxicitytonaturalpopulationsisalwaysquestionedduetotheir
reproductivesurplus.WeusedacometassaytoquantifyprimaryDNAdamageafterexposuretoa
referencemutagenethylmethanesulfonateintwospeciesofcrustaceanwithdifferentreproductive
strategies(sexual Artemia franciscana and asexual Artemia parthenogenetica). Wethenassessedwhether
this predictedindividualperformanceandpopulationgrowthrateoverthreegenerations. Artemia were
exposedtodifferentchronicconcentrations(0.78mM,1.01mM,1.24mMand1.48mM)ofethyl
methanesulfonatefrominstar1onwardsfor3h,24h,7days,14daysand21daysandpercentagetail
DNA valueswereusedforcomparisonsbetweenspecies.ThepercentagetailDNAvaluesshowed
consistentlyelevatedvaluesupto7daysandshowedareductionfrom14daysonwardsin A.
franciscana. Whilstin A. parthenogenetica such areductionwasevidenton21daysassessment.The
values ofpercentagetailDNAafter21dayswerecomparedwithpopulationlevelfitnessparameters,
growth,survival,fecundityandpopulationgrowthratetoknowwhetherprimaryDNAdamageas
measuredbycometassayisareliablebiomarker.SubstantialincreaseintailDNAvalueswasassociated
with substantialreductionsinallthefitnessparametersintheparentalgenerationof A. franciscana and
parental,F1andF2generationsof A. parthenogenetica. Socometresultsweremorepredictiveinasexual
speciesovergenerations.Theseresultspointedtotheimportanceofpredictingbiomarkerresponses
from multigenerationalconsequencesconsideringlifehistorytraitsandreproductivestrategiesin
ecologicalriskassessments.
Not Available
 
Date 2021-09-16T08:12:46Z
2021-09-16T08:12:46Z
2013
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63118
 
Language English
 
Relation Not Available