Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses?
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Title |
Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses?
Not Available |
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Creator |
Sukumaran,Sandhya
Grant,Alastair |
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Subject |
Artemia franciscana
Artemia parthenogenetica Ethyl methanesulfonate Genotoxicity Comet assay Fitness effects |
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Description |
Not Available
The impactofchronicgenotoxicitytonaturalpopulationsisalwaysquestionedduetotheir reproductivesurplus.WeusedacometassaytoquantifyprimaryDNAdamageafterexposuretoa referencemutagenethylmethanesulfonateintwospeciesofcrustaceanwithdifferentreproductive strategies(sexual Artemia franciscana and asexual Artemia parthenogenetica). Wethenassessedwhether this predictedindividualperformanceandpopulationgrowthrateoverthreegenerations. Artemia were exposedtodifferentchronicconcentrations(0.78mM,1.01mM,1.24mMand1.48mM)ofethyl methanesulfonatefrominstar1onwardsfor3h,24h,7days,14daysand21daysandpercentagetail DNA valueswereusedforcomparisonsbetweenspecies.ThepercentagetailDNAvaluesshowed consistentlyelevatedvaluesupto7daysandshowedareductionfrom14daysonwardsin A. franciscana. Whilstin A. parthenogenetica such areductionwasevidenton21daysassessment.The values ofpercentagetailDNAafter21dayswerecomparedwithpopulationlevelfitnessparameters, growth,survival,fecundityandpopulationgrowthratetoknowwhetherprimaryDNAdamageas measuredbycometassayisareliablebiomarker.SubstantialincreaseintailDNAvalueswasassociated with substantialreductionsinallthefitnessparametersintheparentalgenerationof A. franciscana and parental,F1andF2generationsof A. parthenogenetica. Socometresultsweremorepredictiveinasexual speciesovergenerations.Theseresultspointedtotheimportanceofpredictingbiomarkerresponses from multigenerationalconsequencesconsideringlifehistorytraitsandreproductivestrategiesin ecologicalriskassessments. Not Available |
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Date |
2021-09-16T08:13:52Z
2021-09-16T08:13:52Z 2013 |
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Type |
Article
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/63151 |
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Language |
English
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Relation |
Not Available
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