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Isolation and characteriation of extreme halophiles Halomonas aquamarina and Halomonas marina from trigger fish, Abalistes stellaris (Bloch & Schneider, 1801)

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Title Isolation and characteriation of extreme halophiles Halomonas aquamarina
and Halomonas marina from trigger fish, Abalistes stellaris
(Bloch & Schneider, 1801)
Not Available
 
Creator Mathew,Anu
Susmitha,V
Imelda,Joseph
 
Subject Abalistes stellaris
Halophiles
16SrRNA
Halomonas aquamarina
Halomonas marina
 
Description Not Available
Screening of bacteria from the skin, gills and gut of the marine triggerfish, Abalistes stellaris (Bloch & Schneider, 1801),
collected from Vizhinjam, Kerala, India, led to the isolation of 8 halophilic bacterial strains. The isolates were able to grow
optimally in culture media with 5-15% salt content. Of these, 3 extremely halophilic bacterial isolates that grew in 20-25%
of salt were selected for genotypic characterisation. Bacterial strain, IJ1, isolated from skin, and strains, IJ5 and IJ6, isolated
from gut of A. stellaris grew optimally at pH 7.0 and 5-15% NaCl at 35 °C. The cells were Gram negative short rods.
According to the phenotypic characteristics and comparative partial 16SrRNA sequence analysis, the strain IJ1 was identified
as Halomonas marina strain DSM 4741 (GenBank Accession Number: KC599209) and strains IJ5 and IJ6 as Halomonas
aquamarina (GenBank Accession Numbers: KC620376 and KC599210 respectively). H. aquamarina has been reported for
the first time from any environment in India and both the strains have been deposited in the microbial repository of National
Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, India.
Not Available
 
Date 2021-09-16T09:18:00Z
2021-09-16T09:18:00Z
2013
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63182
 
Language English
 
Relation Not Available