Record Details

Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular cloning, characterization and expression analysis of
cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster
Pinctada fucata
Not Available
 
Creator Anju,A
Joseph,Jeswin
Thomas,P C
Paulton,M P
Vijayan,K K
 
Subject Cu/Zn SOD
Pinctada fucata
cDNA cloning
Phylogenetic analysis
gene expression
 
Description Not Available
Because of its capacity to rapidly convert superoxide to hydrogen peroxide, superoxide dismutase (SOD) is
crucial in both intracellular signalling and regulation of oxidative stress. In this paper we report the
cloning of a Cu/Zn SOD (designated as pfSOD) from the pearl oyster (Pinctada fucata) using rapid amplification
of cDNA ends (RACE) PCR. The full-length cDNA of this Cu/Zn SOD contains an open reading frame
(ORF) of 471 bp coding for 156 amino acids. No signal peptide was identified at the N-terminal amino acid
sequence of Cu/Zn SOD indicating that this pfSOD encodes a cytoplasmic Cu/Zn SOD. This is supported by
the presence of conserved amino acids required for binding copper and zinc. Semi-quantitative analysis in
adult tissues showed that the pfSOD mRNA was abundantly expressed in haemocytes and gill and scarcely
expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfSOD
mRNA in haemocytes was increased, reaching the highest level at 8 h, then dropping to basal levels at
36 h. These results suggest that Cu/Zn SOD might be used as a bioindicator of the aquatic environmental
pollution and cellular stress in pearl oyster.
Not Available
 
Date 2021-09-16T09:18:03Z
2021-09-16T09:18:03Z
2013
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63203
 
Language English
 
Relation Not Available