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Effect of depuration of animals and use of antimicrobial agents on proliferation of cells and microbial contamination in in-vitro mantle explant culture of the abalone Haliotis varia Linnaeus

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Title Effect of depuration of animals and use of antimicrobial agents on
proliferation of cells and microbial contamination in in-vitro mantle
explant culture of the abalone Haliotis varia Linnaeus
Not Available
 
Creator Meena,Anu@
Dharmaraj,S
Sukumaran,N
Suja,C P
 
Subject Abalone
Cell proliferation
Depuration
Explant culture
In-vitro pearl
 
Description Not Available
The role played by depuration of test animals and surface sterilisation of tissues has been greatly felt in tissue cultures of
molluscs so as to control contamination and to ensure viable cultures. The effect of depuration and the optimum dose of
antimicrobial agents for sterilisation of mantle tissue in abalone mantle tissue culture and their effects in cell yield have not
been reported with reference to cell proliferation and its survival. Abalone cell culture is being given importance in order
to develop a technology for in-vitro pearl production, as the cultured pearls are highly valuable in terms of high quality.
Separate studies were conducted to evaluate the effect of depuration and to standardise the optimum dose of antimicrobial
agents. Mantle tissue from depurated and non-depurated abalones (Haliotis varia) was used simultaneously to study
the effect of depuration in cell yield. During the study, contamination was found to be more in depurated tissues than in
non-depurated ones. Role of antibiotics such as streptomycin and penicillin on cell proliferation and contamination was
studied by washing tissues using solutions containing three different doses of antibiotics, prior to initiation of culture. In
the first experiment, washing of tissue in seawater containing 1000 μg ml-1 of streptomycin and 2000 IU ml-1 of penicillin
yielded more cell proliferation with less contamination. Fungizone was incorporated along with streptomycin and penicillin
to minimise the fungal contamination in the second experiment. The dosage of 200 μg ml-1 of fungizone with above said
antibiotic dosage yielded better cell proliferation and less contamination than other doses.
Not Available
 
Date 2021-09-17T04:07:08Z
2021-09-17T04:07:08Z
2014
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63488
 
Language English
 
Relation Not Available
 
Publisher Central Marine Fisheries Research Institute