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Polyketide Family of Novel Antibacterial 7‑O‑Methyl-5′-hydroxy-3′- heptenoate−Macrolactin from Seaweed-Associated Bacillus subtilis MTCC 10403

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Title Polyketide Family of Novel Antibacterial 7‑O‑Methyl-5′-hydroxy-3′-
heptenoate−Macrolactin from Seaweed-Associated Bacillus subtilis
MTCC 10403
Not Available
 
Creator Thilakan,Bini
Raola,Vamshi Krishna
Chakraborty,Kajal
 
Subject seaweed, marine bacteria, antibacterial activity, polyketide synthase, 7-O-methyl-5′-hydroxy-3′-heptenoate−macrolactin
 
Description Not Available
Seaweed-associated heterotrophic bacterial communities were screened to isolate potentially useful antimicrobial
strains, which were characterized by phylogenetic analysis. The bacteria were screened for the presence of metabolite genes
involved in natural product biosynthetic pathway, and the structural properties of secondary metabolites were correlated with the
genes. Bioactivity-guided isolation of polyene antibiotic 7-O-methyl-5′-hydroxy-3′-heptenoate−macrolactin from Bacillus subtilis
MTCC10403 associated with seaweed Anthophycus longifolius using mass spectrometry and extensive 2D-NMR studies was
carried out. The newly isolated macrolactin compound is a bactericidal antibiotic with broad spectrum activity against human
opportunistic clinical pathogens. The biosynthetic pathway of 7-O-methyl-5′-hydroxy-3′-heptenoate−macrolactin by means of a
stepwise, decarboxylative condensation pathway established the PKS-assisted biosynthesis of the parent macrolactin and the sidechain
5-hydroxyhept-3-enoate moiety attached to the macrolactin ring system at C-7. Antimicrobial activity analysis combined
with the results of amplifying genes encoding for polyketide synthetase and nonribosomal peptide synthetase showed that
seaweed-associated bacteria had broad-spectrum antimicrobial activity. The present work may have an impact on the exploitation
of macrolactins for pharmaceutical and biotechnological applications.
Not Available
 
Date 2021-09-17T04:07:22Z
2021-09-17T04:07:22Z
2014
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63579
 
Language English
 
Relation Not Available
 
Publisher American Chemical Society