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Evaluation of Various Tissues of the Caerulean Damsel, Pomacentrus caeruleus for Initiating In Vitro Cell Culture Systems

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Title Evaluation of Various Tissues of the Caerulean Damsel,
Pomacentrus caeruleus for Initiating In Vitro Cell Culture
Systems
Not Available
 
Creator Sheethal,Mary Sunny
Sobhana,K S
George,Githa Ann
Sreedevi,S
 
Subject Caudal peduncle
Fin
Liver
Marine ornamental fish cell lines
Pomacentrus caeruleus
 
Description Not Available
Explantation and trypsinisation methods for tissue
dissociation were attempted for the establishment of
primary cell cultures from the caerulean damsel, Pomacentrus
caeruleus. Among the tissues taken, fin, liver and
caudal peduncle showed good attachment with emergence
of cells. The cells were best suited to grow in Leibovitz’s
L-15 basal medium supplemented with foetal bovine serum
(initially 20 % which was later reduced to 5–10 % during
subsequent passages) at an ambient temperature of
28 ± 2 �C and pH 7.2 ± 0.2. These cultures persisted at
temperatures from 17 to 32 �C, and proliferated at temperatures
from 24 to 30 �C. The cells have been cryopreserved
successfully with a survival rate of 80 %. Results
suggest that fin, caudal peduncle and liver cell cultures
have potential for development into cell lines.
Not Available
 
Date 2021-09-17T05:37:27Z
2021-09-17T05:37:27Z
2016
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63682
 
Language English
 
Relation Not Available
 
Publisher National Academy of Sciences, India