Record Details

Antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium Bacillus subtilis MTCC 10403

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Antimicrobial polyketide furanoterpenoids from seaweed-associated
heterotrophic bacterium Bacillus subtilis MTCC 10403
Not Available
 
Creator Thilakan,Bini
Raola,Vamshi Krishna
Chakraborty,Kajal
 
Subject Anthophycus longifolius
Sargassaceae
Seaweed
Bacillus subtilis MTCC 10403
Antibacterial activity
Polyketide furanoterpenoids
Polyketide synthase
 
Description Not Available
Brown seaweed Anthophycus longifolius (Turner) Kützing (family Sargassaceae) associated heterotrophic
bacterium Bacillus subtilis MTCC 10403 was found to be a potent isolate with broad range of antibacterial
activity against important perceptive food pathogens Vibrio parahaemolyticus, V. vulnificus, and Aeromonas
hydrophila. This bacterium was positive for polyketide synthetase gene (KC589397), and therefore,
was selected to bioprospect specialized metabolites bearing polyketide backbone. Bioactivity-guided
chromatographic fractionation of the ethyl acetate extract of the seaweed-associated bacterium segregated
four homologous polyketide furanoterpenoids with potential antibacterial activities against clinically
important pathogens. The minimum inhibitory concentration (MIC) assay showed that the referral
antibiotics tetracycline and ampicillin were active at 25 mg/mL against the test pathogens, whereas the
previously undescribed (4E)-methyl 13-((16-(furan-2-yl) ethyl)-octahydro-7-hydroxy-4-((E)-23-
methylbut-21-enyl)-2H-chromen-6-yl)-4-methylpent-4-enoate (compound 1) and methyl 3-(hexahydro-
9-((E)-3-methylpent-1-enyl)-4H-furo[3,2-g]isochromen-6-yl) propanoate (compound 3) displayed
antibacterial activities against the test pathogens at a lesser concentration (MIC < 7 mg/mL). The title
compounds were characterized by comprehensive nuclear magnetic resonance and mass spectroscopic
experiments. Polyketide synthase catalyzed putative biosynthetic mechanism additionally corroborated
the structural ascriptions of the hitherto undescribed furanoterpenoids from seaweed-associated bacterial
symbiont. The electronic and hydrophobic parameters appeared to hold a conspicuous part in
directing the antibacterial properties of the compounds. Seaweed-associated B. subtilis MTCC 10403
demonstrated to represent a potential source of antimicrobial polyketides for pharmaceutical
applications.
Not Available
 
Date 2021-09-17T07:57:25Z
2021-09-17T07:57:25Z
2017
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63771
 
Language English
 
Relation Not Available
 
Publisher Not Available