Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus Not Available |
|
Creator |
Joseph,Dexy
Chakraborty,Kajal |
|
Subject |
C20-22 polyunsaturated fatty acids deep sea leafscale gulper shark Centrophorus squamosus winterization urea complexation argentation chromatography |
|
Description |
Not Available
The C20-22 polyunsaturated fatty acids (C20-22 PUFAs), mainly eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), were concentrated from the refined liver oil of deep sea leafscale gulper shark, Centrophorus squamosus, by sequential processes of winterization, urea complexation, and argentation chromatography. Winterization at 4°C using acetonitrile as solvent showed significant reduction of the total saturated fatty acid content (12%) with a concomitant increase of polyunsaturated fatty acids (36.3%). The urea complexation process significantly enriched the total polyunsaturated fatty acids (49.5%), with a reduction in saturated fatty acids (83.9%). Silica gel based argentation chromatography further concentrated the esters of C20-22 PUFA (>99 percent purity). Nuclear Magnetic Resonance spectroscopy was used as a chemical fingerprinting tool to study the enrichment of C20-22 PUFAs at various stages of the purification process. An increase in the proton signal intensity at the olefinic region (at δ 4–6) and a decrease in the aliphatic signals (at δ 0.5–2) showed that the process successfully enriched the C20-22 PUFAs. Not Available |
|
Date |
2021-09-17T07:59:38Z
2021-09-17T07:59:38Z 2017 |
|
Type |
Article
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/63845 |
|
Language |
English
|
|
Relation |
Not Available
|
|
Publisher |
Elsevier
|
|