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Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus

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Title Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined
Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus
Not Available
 
Creator Joseph,Dexy
Chakraborty,Kajal
 
Subject C20-22 polyunsaturated fatty
acids
deep sea leafscale
gulper shark
Centrophorus
squamosus
winterization
urea complexation
argentation chromatography
 
Description Not Available
The C20-22 polyunsaturated fatty acids (C20-22 PUFAs), mainly eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), were concentrated from the refined liver oil of deep sea leafscale gulper shark, Centrophorus squamosus, by sequential processes of winterization, urea complexation, and argentation chromatography. Winterization at 4°C using acetonitrile as solvent showed significant reduction of the total saturated fatty acid content (12%) with a concomitant increase of polyunsaturated fatty acids (36.3%). The urea complexation process significantly enriched the total polyunsaturated fatty acids (49.5%), with a reduction in saturated fatty acids (83.9%). Silica gel based argentation chromatography further concentrated the esters of C20-22 PUFA (>99 percent purity). Nuclear Magnetic Resonance spectroscopy was used as a chemical fingerprinting tool to study the enrichment of C20-22 PUFAs at various stages of the purification process. An increase in the proton signal intensity at the olefinic region (at δ 4–6) and a decrease in the aliphatic signals (at δ 0.5–2) showed that the process successfully enriched the C20-22 PUFAs.
Not Available
 
Date 2021-09-17T07:59:38Z
2021-09-17T07:59:38Z
2017
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/63845
 
Language English
 
Relation Not Available
 
Publisher Elsevier